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Human peripheral blood mononuclear cells (PBMCs) were fixed and permeabilized using the Cell Signaling Buffer Set A followed by intracellular staining with Anti-SLP-76 antibodies. Flow cytometry was performed with the MACSQuant®
Analyzer. The images show pregated lymphocyte subpopulations that were selected based on scatter signals and surface antigen expression: CD3+
T cells (left image), CD3-
B cells (center image) and CD3-
NK cells (right image). Cell debris were excluded from the analysis based on scatter signals.
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