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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (right peak) or stimulated with 100 ng/mL phorbol 12-myristate 13-acetate (PMA) for 24 hours. The cells were fixed and permeabilized with the Cell Signaling Buffer Set A and stained with Anti-PKCα antibodies. Cells were then analyzed by flow cytometry using the MACSQuant®
Analyzer. Lymphocytes were pre-gated for the analysis. Cell debris were excluded from the analysis based on scatter signals. As shown in the histogram, PMA stimulation of lymphocytes results in decreased PKCα expression.