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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Human PBMCs were either left unstimulated (left image) or stimulated with Treg Expansion CD3/CD28 MACSiBead Particles for 48 hours. The cells were stained with Anti-LAP (TGF-β1) antibodies. LAP-TGF-β1–staining was controlled by incubating cells with unconjugated Anti-LAP (TGF-β1) antibodies prior to labeling with fluorochrome-conjugated antibodies. After that the cells were fixed, permeabilized, and intracellularly stained with Anti-FoxP3‑APC or -PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Gating was performed according to the CD4 expression and side scatter properties of the cells. Cell debris was excluded from the analysis in an fluorescence 3 versus fluorescence 4 dot plot.
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