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Miltenyi Biotec distribution:
Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left peak) or stimulated with LPS for 6 hours. After two hours 1 µg/mL brefeldin A was added. Cells were then fixed, permeabilized, and stained with Anti-IL-8 antibodies as well as with CD14 antibodies. CD14+
cells were pre-gated for the analysis. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.