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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Mouse spleen cells were incubated with or without PMA/Ionomycin for 6 hours. After 2 hours, brefeldin A was added. The cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IL-5-PE, CD4-FITC, and CD154-APC. Cells were analyzed by flow cytometry using the MACSQuant® Analyzer. Gating was performed according to the CD4 expression and side scatter properties of the cells.
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