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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Mouse spleen cells were incubated with or without KLH for 6 hours. After 2 hours, brefeldin A was added. Cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IFN-γ antibodies and CD154 antibodies. Cells were analyzed by flow cytometry. Gating was performed according to CD4 expression and side scatter properties of the cells.