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Cells from the mouse pre–B cell 1881 cell line were stably transfected with the H-2Kk
gene and mixed with untransfected cells from this cell line in a 1:3 ratio. The mixed cells were stained with Anti-H-2Kk
antibodies conjugated to FITC (A), -PE (B), -APC (C), -PE-Vio770 (D), or -APC-Vio770 (E) and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of Anti-H-2Kk
-PE-Vio770 and Anti-H-2Kk
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