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Spleen cells from DO11.10 transgenic mice were stained with Anti-DO11.10 TCR antibodies conjugated to (A) FITC (B) PE (C) APC (D) VioBlue as well as with CD4-PE or CD4-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells labeled with Anti-DO11.10 TCR-Biotin (E) were stained with Anti-Biotin-PE as well as CD4-APC. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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