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myeloid dendritic cells were isolated from human PBMCs using the CD1c (BDCA-1)+
Dendritic Cell Isolation Kit, an LD Column, two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator. Cells were fluorescently stained with CD1c (BDCA-1)-PE, CD14-APC, or CD19-VioBright™ 515. Cell debris and dead cells were excluded from the analysis based on scatter signals and prodipium iodide fluorescence and erythrocytes based on CD45-negativity.
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