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PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Subsequently, IL-13-secreting cells were enriched and analyzed for IL-13 secretion and expression of CD154 using the IL-13 Secretion Assay - Cell Enrichment and Detection Kit, and CD154-APC. The plots show stimulated and unstimulated cells before and after separation. CD154 expression and IL-13 secretion by viable CD4+
T cells is shown.
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