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Rat neonatal hearts were dissociated using the Neonatal Heart Dissociation Kit, mouse and rat (# 130-098-373) in combination with the gentleMACS Octo Dissociator with Heaters. Subsequently, neonatal cardiac fibroblasts were isolated using the Cardiac Fibroblast Isolation Kit, rat and cultured in DMEM medium with 10 FBS and 100 U/mL penicillin/100 U/mL streptomycin for 1 day.
Immunofluorescence pictures show labeling of cardiac fibroblasts with an anti-Prolyl 4-hydroxylase β antibody (green) before (A) and after (B) magnetic enrichment using the Neonatal Cardiac Fibroblast Isolation Kit, rat. Nuclei are visualized by DAPI staining (blue).