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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
In September of 2016, Miltenyi Biotec started to offer flow cytometry antibodies in a concentrated format of 2 μL of antibody per test*. Due to an increasing demand for smaller pipetting volumes for multicolor panels with many antibodies, we are glad to introduce the change from a 10 μL of antibody/test to a 2 μL of antibody/test format for many of our antibodies. The change will be accompanied by a change from 5 mL amber glass bottles to 2 mL polypropylene vials, which will be marked with color-coded labels for easy identification and organization of your flow reagent inventory.
From September 2016 onwards, all newly released products are available in this concentrated format. All existing products available in the 10 μL of antibody/test format will continue to be offered for sale until inventories are depleted. Upon new inventory manufacture, you will begin receiving products provided in the more concentrated, 2 μL of antibody/test format. Be sure to check the up-to-date datasheet to confirm the correct volume per test.
*One test corresponds to labeling of up to 106 cells in a total volume of 100 μL.
Best in class titer!
Our 2 mL vial format provides you with highly concentrated antibodies. This requires you to only use 2 μL of antibody volume per test, which means more flexibility and more antibody specificities per test, allowing you to expand your multicolor panels.
A pipetting volume of 2 μL per test reduces the total antibody volume in a multicolor staining mix. This allows that even in multicolor panels with up to 25 antibodies, the total antibody volume does not exceed 50% of the total staining volume.
| Number of antibodies in a multicolor panel | Total staining volume per test (μL) | Antibody volume per test (μL) | Remaining buffer volume (μL) |
|---|---|---|---|
| 3 | 100 | 6 | 94 |
| 4 | 100 | 8 | 92 |
| 5 | 100 | 10 | 90 |
| 6 | 100 | 12 | 88 |
| 7 | 100 | 14 | 86 |
| 8 | 100 | 16 | 84 |
| 12 | 100 | 24 | 76 |
| 15 | 100 | 30 | 70 |
| 20 | 100 | 40 | 60 |
| 25 | 100 | 50 | 50 |
Why color-coded labels?
Color-coding means easy identification and organization of your reagents. Organize your flow reagent inventory according to the emission color of conjugates, the laser used for excitation of fluorochromes, and/or according to the name of the antigen. The color-coded labels of our 2 mL polypropylene vials indicate the color closest to the emission color of different conjugates and the corresponding laser excitation color. In addition, all 2 mL vials have bright orange caps with colored labels that conveniently correspond to the emission color of the conjugate.
Pick your size!
In order to provide you with convenient choices for setting-up your large scale and high-throughput experiments, we are introducing bulk-sized options for selected products. For additional large-scale sizes and available discounts for our products visit Custom Antibody Design Services.
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