Recombinant Human IL-12 (interleukin 12) can promote proliferation of NK and T cells, regulates their cytotoxic activity, and can induce T
H
1 differentiation of T cells. The T cell-stimulating factor IL-12 is a crucial regulator of cell mediated immune responses as it also induces the production of IFN-γ by PBMCs, among other cytokines. The recombinant protein is manufactured for use in differentiation studies, cell culture, and functional assays.

Data and images for Human IL-12 (CHO)

Figures

Figure 1

View details
Human IL-12 (CHO) activity assay.
The biological activity of Human IL-12 (CHO) is determined by induction of IFN-γ secretion by PHA-activated T cells.

Figure 1

Human IL-12 (CHO) activity assay.
The biological activity of Human IL-12 (CHO) is determined by induction of IFN-γ secretion by PHA-activated T cells.

Figure 2

View details
SDS-PAGE of Human IL-12 (CHO), premium grade
under reducing (R) and non-reducing (NR) conditions.

Figure 2

SDS-PAGE of Human IL-12 (CHO), premium grade
under reducing (R) and non-reducing (NR) conditions.

Specifications for Human IL-12 (CHO)

Overview

Recombinant Human IL-12 (interleukin 12) can promote proliferation of NK and T cells, regulates their cytotoxic activity, and can induce T
H
1 differentiation of T cells. The T cell-stimulating factor IL-12 is a crucial regulator of cell mediated immune responses as it also induces the production of IFN-γ by PBMCs, among other cytokines. The recombinant protein is manufactured for use in differentiation studies, cell culture, and functional assays.

Applications

Human IL-12 (CHO) can be used for a variety of applications, including:
  • In vitro differentiation of naive CD4+ T cells towards TH1 cells.
  • In vitro proliferation and stimulation of cytotoxic activity of NK cells and T cells.

Detailed product information

Background information

Interleukin 12 (IL-12) is a heterodimeric proinflammatory cytokine and a modulator of cell-mediated immunity, which is mainly produced by macrophages, dendritic cells, and B cells. IL-12 stimulates the production and secretion of several cytokines, in particular IFN-γ, by NK cells and T cells, induces proliferation, and enhances the cytotoxic activity within these cell populations. Another important activity of IL-12, acting together with IFN-γ and IL-2, is to drive T helper cell responses toward the T
H
1 rather than the T
H
2 phenotype. Moreover, IL-12 is also important in resistance to viral disease and has significant antitumor activity. It has been shown that single chain fusion proteins of naturally occurring heterodimeric cytokines such as IL-12 or IL-23 are bioactive
in vitro
and
in vivo
.

Biological activity

  • Induction of IFN-γ secretion by human T cells (NIBSC 95/544)
  • research grade: ≥ 1.5×
    10
    6
    U/mg
  • premium grade: ≥ 3×
    10
    6
    U/mg
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human IL-12 (CHO)

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human IL-12 (CHO)

Publications

  1. Wulff, H. et al. (2007) Cloning and characterization of an adenoviral vector for highly efficient and doxycycline-suppressible expression of bioactive human single-chain interleukin 12 in colon cancer. BMC Biotechnol. 7: 35

Related products for
Human IL-12 (CHO)

7 products available | view all

Seems like you are coming from USA!
Do you want to visit our website in your country?