Clone:
REA1244
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
GP40, Leu-9, TP41, Tp40

Extended validation for CD7 Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD7 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD7-PE (REA1244, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD7 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD7-PE (REA1244, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD7 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD7-PE (REA1244, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD7 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD7-PE, clone (REA1244). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD7 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD7-PE, clone (REA1244). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD7. Human peripheral blood mononuclear cells (PBMCs) were stained with CD7 antibodies and with a suitable counterstaining. As a control, CD7 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD7. Human peripheral blood mononuclear cells (PBMCs) were stained with CD7 antibodies and with a suitable counterstaining. As a control, CD7 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD7. Human peripheral blood mononuclear cells (PBMCs) were stained with CD7 antibodies and with a suitable counterstaining. As a control, CD7 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD7. Human peripheral blood mononuclear cells (PBMCs) were stained with CD7 antibodies and with a suitable counterstaining. As a control, CD7 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD7. Human peripheral blood mononuclear cells (PBMCs) were stained with CD7 antibodies and with a suitable counterstaining. As a control, CD7 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD7. Human peripheral blood mononuclear cells (PBMCs) were stained with CD7 antibodies and with a suitable counterstaining. As a control, CD7 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD7. Human peripheral blood mononuclear cells (PBMCs) were stained with CD7 antibodies and with a suitable counterstaining. As a control, CD7 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD7. Human peripheral blood mononuclear cells (PBMCs) were stained with CD7 antibodies and with a suitable counterstaining. As a control, CD7 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD7. Human peripheral blood mononuclear cells (PBMCs) were stained with CD7 antibodies and with a suitable counterstaining. As a control, CD7 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD7. Human peripheral blood mononuclear cells (PBMCs) were stained with CD7 antibodies and with a suitable counterstaining. As a control, CD7 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD7 (REA1244). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD7 (REA1244). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD7 (REA1244). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD7 Antibody, anti-human, REAfinity™

Overview

Clone REA1244 recognizes the human CD7 antigen, a single-pass type I membrane protein, also known as leu-9 or GP40. CD7 appears early in T cell ontogeny and is expressed by most T cells in the periphery. It is also found on NK cells, hematopoietic progenitors, monocytes, acute lymphocytic leukemia, and some acute myeloid leukemia cells. Although the ligand is yet not identified, CD7 has been recognized as a costimulatory molecule. CD7 activates phosphatidylinositol 3-kinase which is involved in CD7-mediated regulation of integrin adhesiveness. Furthermore, it has been reported that the β-galactoside-binding lectin galectin-1 binds CD7, leading to induction of apoptosis of thymocytes and T cells, with implications for certain autoimmune diseases and T cell lymphomas.
Additional Information: Clone REA1244 displays negligible binding to Fc receptors.

Alternative names

GP40, Leu-9, TP41, Tp40

Detailed product information

Technical specifications

CloneREA1244
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD7
Alternative names of antigenGP40, Leu-9, TP41, Tp40
Molecular mass of antigen [kDa]23
Distribution of antigenNK cells, T cells, stem cells, other
Entrez Gene ID924
RRIDAB_2819708, AB_2819718, AB_2819709, AB_2819719, AB_2819710, AB_2819720, AB_2819711, AB_2819721, AB_2819712, AB_2819722, AB_2819713, AB_2819723, AB_2819714, AB_2819724, AB_2819715, AB_2819725, AB_2819716, AB_2819717

Resources for CD7 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD7 Antibody, anti-human, REAfinity™

Publications

  1. Aruffo, A. et al. (1987) Molecular cloning of two CD7 (T-cell leukemia antigen) cDNAs by a COS cell expression system. EMBO J. 6(11): 3313-3316
  2. Gallacher, L. et al. (2000)
    Isolation and characterization of human CD34
    Lin
    and CD34
    +
    Lin
    hematopoietic stem cells using cell surface markers AC133 and CD7.
    Blood 95(ARVO Annual Meeting Abstract): 2813-2820
  3. Kobayashi, S. et al. (2013) The CD3 versus CD7 plot in multicolor flow cytometry reflects progression of disease stage in patients infected with HTLV-I. PLoS One 8(1): e53728

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