Research solutions for exosome isolation and analysis

Exosomes belong to the group of extracellular vesicles (EV) and play a key role in cellular functions. Consequently, they are of great interest in basic research, as well as in future diagnostic applications and/or clinical settings. Our specific solutions enable you to carry out exosome isolation without any density gradient centrifugation. It is possible to target CD9, CD63, CD81, or all markers combined. On top, we offer a flow cytometry assay for fast exosome screening.

 

Exosome isolation

Exosomes belong the to group of extracellular vesicles (EV) and play a key role in cellular functions. Consequently, they are of great interest in basic research as well as in future diagnostic applications or clinical settings. We offer a reliable isolation method that safes time, capital equipment, and high starting volumes (50–100 mL) – a true alternative for conventional ultracentrifugation.

Isolation of extracellular vesicles

Isolation of EVs using MACS® Technology

Using our Exosome Isolation Kit, enrichment of EVs has never been easier. 

  • Selection of EVs according to surface markers i.e. CD9, CD63, or CD81
  • Low starting volume of samples (0.5 to 2 mL)
  • Isolation from multiple sample types e.g. cell culture supernatant, urine, plasma, serum, ascites etc.
Western Blot analysis of NK cell EVs and platelet EVs

Separation of EV subpopulations

Subpopulations of EVs can be separated using superparamagnetic MACS® MicroBeads. For example, NK cell EVs and platelet EVs can be separated using CD81+ and CD9+ MicroBeads.

Required products

Exosome isolation kits

Related PDFs

Isolation of highly pure urinary extracellular vesicles suitable for downstream RNA cargo sequencing (application note)
Full workflow to isolate and analyze extracellular vesicles released during physical exercise (application note)
Diversity of extracellular vesicles addressed by immunoaffinity beads (scientific poster)
MACSPlex Analysis of plasma- derived extracellular vesicles (application note)
 

Exosome analysis

For further downstream analysis of exosomes we have established the MACSPlex Exosome Assay. This multiplex bead-based assay consists of capture and detection antibodies to analyze the composition of exosome surface proteins in a given sample by flow cytometry.

Workflow of the multiplex bead platform

Advantages of the MACSPlex Exosome Assay

Comprehensive – Fast and easy screening of 39 surface markers on EVs

Flexible – Additional antibodies can be added for multi-parameter analysis

Surface marker profile of EVs isolated from melanoma cell cultures or colon cancer cells

Discrimination of EVs by differing tumor markers

EVs isolated from cancer cell lines can be discriminated by differing markers from tumor entities. EVs from melanoma cell line for instance show different surface markers then EVs from colon cancer cells as demonstrated by binding to the respective MACSPlex Exosome capture beads.

Required products

MACSPlex Exosome Kit, human

Related PDFs

Robust EV profiling in various sample types with the MACSPlex Exosome Kit (application note)
Customization of MACSPlex Exosome Kit enables comprehensive, tailored EV analysis (application note)
General instructions for MACSPlex Exosome Kit (application note)
A multiplex bead platform for protein profiling of exosomes by flow cytometry (scientific poster)

Related videos

Exosome analysis using a multiplex bead-based flow cytometry assay (webinar)
video preview
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Seminar
Surface Protein Characterization of Exosome Nanoparticles using a Multiplexing Approach

Join John W. Ludlow, Ph.D., Executie Director of ZenBio Inc., and his talk at the AACR annual meeting 2017.

 

Step-by-step protocols for exosome research

Get acquainted with our protocols for the isolation and analysis of exosomes in cancer research.

Matching:

Isolation and analysis of human exosomes

  • Keywords
  • Molecular applications
  • Flow cytometry and cell analysis
  • human
  • Application protocol

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