MBTP 22: Immunophenotyping of human pluripotent stem cells (PSCs) using flow cytometry

Miltenyi Biotec-tested panel 22 (MBTP 22)

This application protocol describes the quantitative analysis of human pluripotent stem cell (PSC) differentiation potential using flow cytometry. Human PSCs can easily be differentiated using the StemMACS™ Trilineage Differentiation Kit which enables directed and reproducible differentiation into ectodermal, mesodermal, and endodermal lineages ready for downstream applications, such as flow cytometric analysis.

Protocol

Materials

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