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Structures of the central nervous system and their functions
| CNS structure | Organization/substructures | Functions |
| Cerebral hemispheres | Corpus callosum | Connects left and right cerebral hemispheres |
Outer layer: Cerebral cortex | The largest region of the mammalian brain is divided into the frontal, parietal, temporal, and occipital lobes. Responsible for various functions, such as cognition, memory, awareness, consciousness, language, movement. | |
Inner substructures: Amygdala | Perception and communication of emotions | |
| Hippocampus | Memory formation | |
| Basal ganglia | Regulation of movement | |
| Diencephalon | Thalamus | Process most information input to cerebral cortex |
| Hypothalamus | Regulation of autonomic, endocrine and visceral functions | |
| Midbrain | Sensory and motor function control | |
| Substantia nigra | Closely associated with motor system pathways of basal ganglia | |
| Cerebellum | Connected to the brain stem. Modulates movement and is involved in learning motor skills | |
| Pons | Conveys information about movement from cerebral hemispheres to cerebellum | |
| Medulla oblongata | Regulates vital autonomic functions like breathing, blood pressure, heart rate | |
| Spinal cord | Signal transmission from motor cortex to body, and from sensory neurons to sensory cortex; reflex center |
MACS Handbook:
Neural cells (mouse)
MACS Handbook:
Neural cells (mouse)
At a glance: Kits, reagents, and hardware for the preparation of neural tissue samples
| Use | Comments | Product |
|---|---|---|
| Kits and reagents | ||
| Dissociation of neural tissue into a single-cell suspension, free of debris and red blood cells (RBC) | Animal age >P7; uses an optimized enzyme mix for enzymatic dissociation and contains debris and RBC removal solutions | Adult Brain Dissociation Kit, mouse and rat |
| Dissociation of neural tissue into a single-cell suspension | Animal age ≤P7; uses papain for enzymatic dissociation | Neural Tissue Dissociation Kit (P) |
| Dissociation of neural tissue into a single-cell suspension | Animal age ≤P7; uses trypsin for enzymatic dissociation | Neural Tissue Dissociation Kit (T) |
| Dissociation of neural tissue into a single-cell suspension optimized for subsequent neuron isolation | Animal age ≤P7 | Neural Tissue Dissociation Kit – Postnatal Neurons |
| Dissociation of brain tumor tissue | Uses papain for enzymatic dissociation | Brain Tumor Dissociation Kit (P) |
| Dissociation of cultured neurospheres | Uses papain for enzymatic dissociation | Neurosphere Dissociation Kit (P) |
| Dissociation of embryoid bodies generated in vitro | Embryoid Body Dissociation Kit, human and mouse | |
| Removal of myelin | To be used on single-cell suspensions | Myelin Removal Beads II, human, mouse, rat |
| Hardware and consumables | ||
| Tissue dissociation | Provides pre-set programs to dissociate different tissues with Miltenyi Biotec's enzyme kits for high cell yield and viability. | gentleMACS™ Octo Dissociator with Heaters |
| Tissue dissociation | Precision tool coupled with gentleMACS Dissociators that generates optimal shearing to efficiently disrupt tissue while keeping cells intact. | gentleMACS C Tube |
| Filtration | Smart design prevents clogging, enables one-step filtering with decreasing filter sizes, and accommodates 50 mL or 15 mL tubes. | MACS® SmartStrainer, 70 µm |
Tissue dissociation into single-cell suspensions with high cell viability and a minimum of cell debris is a prerequisite for reliable cell separation, cell culture, flow cytometry analysis, and molecular analysis. A dissociation procedure that combines optimized enzymatic treatment with gentle mechanical dissociation is crucial for the preparation of viable single-cell suspensions. In case of adult neural tissue, sample preparation must also include removal of debris and erythrocytes to enable effective cell isolation and culture.
Miltenyi Biotec offers standardized, automated dissociation procedures that facilitate fast and reproducible dissociation of neural tissue at all development stages. All procedures are designed to deliver high numbers of viable cells for optimal downstream processing.
A special protocol for dissociating inflamed neural tissue can be downloaded from the Related Resources Panel to the right. MACS Handbook:
Sample preparation
Related PDFs:
Gentle adult brain dissociation (brochure)
Dissociation of inflamed neural tissue using the Multi Tissue Dissociation Kit 1 (special protocol)
Efficient isolation of viable primary neural cells from adult murin brain tissue based on a novel automated tissue dissociation protocol (scientific poster)
MACS® Sample Preparation Standardize your tissue dissociation and homogenization (brochure)
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