Cookie Settings
We use cookies to provide the best possible website experience for you. This includes cookies that are technically required to ensure a proper functioning of the website, as well as cookies which are used solely for anonymous statistical purposes, for more comfortable website settings, or for displaying personalized content. You are free to choose the categories you would like to permit. Please note that depending on your settings, the full functionality of the website may no longer be available. Further information can be found in our Privacy Statement and Cookie Statement.
Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. |
Staining 1 |
PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™Figure 2Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. | |
Staining 2 |
PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™Figure 2Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. | PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™Figure 2Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. |
PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™Figure 2Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. |
Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. |
Staining 1 |
PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™Figure 2Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. | |
Staining 2 |
PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™Figure 2Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. | PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™Figure 2Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. |
PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™Figure 2Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. |
Seems like you are coming from USA!
Do you want to visit our website in your country?
Copyright © 2023 Miltenyi Biotec and/or its affiliates. All rights reserved.