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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
PBMCs of a CMV+
donor were stimulated for 4 hours with CMV lysate. The responding cells were stained and isolated according to secretion of TNF-α using the TNF-α Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 9390 TNF-α-secreting CD4+
T cells were enriched per 106
T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, no TNF-α-secreting CD4+
T cells were detected per 106
T cells after enrichment.
* Percentage represents frequency among CD4+ T cells.
** Percentage represents frequency among enriched cells.