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Splenocytes of BALB/c mice were either left unstimulated (left images) or stimulated with CD3/CD28 antibodies. Cells were then stained with CD223 antibodies as well as with CD3ε antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.