CD223 antibodies, human

CD223 antibodies, human

Clone: REA351 | Dilution: 1:11
Clone REA351 recognizes the human CD223 antigen, a single-pass type I membrane protein also known as lymphocyte activation gene 3 protein (LAG-3). CD223 is a CD4 homolog with four extracellular Ig superfamily domains. Similar to CD4, CD223 oligomerizes at the surfaces of T cells and binds to MHC II on antigen presenting cells but with significantly higher affinity than CD4. CD223 is expressed on activated CD4
+
and CD8
+
T lymphocytes where it associates with the CD3–TCR complex at the cell surface and negatively regulates signal transduction. As a consequence, it negatively regulates T cell proliferation, function, and homeostasis. There is also increasing evidence of its involvement in regulatory function of tumor-infiltrated T cells in cancer, such as in Hodgkin’s lymphomas and in prostate cancer. CD223 is also expressed on activated NK cells, B cells, and plasmacytoid dendritic cells.
Additional information: Clone REA351 displays negligible binding to Fc receptors.

Alternative names

LAG-3, Protein FDC

Technical specifications

  • Antigen: CD223
  • Clone: REA351
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: LAG-3, Protein FDC
  • Entrez Gene ID: 3902
  • Molecular mass of antigen [kDa]: 54
  • Distribution of antigen: T cells, lymphocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE
  • Selected references

    1. Huang, C. T. et al. (2004) Role of LAG-3 in regulatory T cells. Immunity 21(4): 503-513
    2. Triebel, F. et al. (1990) LAG-3, a novel lymphocyte activation gene closely related to CD4. J. Exp. Med. 171(5): 1393-1405
    3. Gagliani, N. et al. (2013) Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells. Nat. Med. 19(6): 739-746
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