CD14 antibodies, human

CD14 antibodies, human

Clone: TÜK4 | Dilution: 1:50
Clone TÜK4 recognizes the human CD14 antigen and cross-reacts with non-human primate CD14. The CD14 antigen is a high affinity receptor for lipopolysaccharides (LPS) and LPS–binding protein (LBP)–complexes. It is part of the functional heteromeric LPS receptor complex comprised of CD14, TLR4, and MD-2.
CD14 is strongly expressed on most human monocytes and macrophages in peripheral blood, other body fluids, and various tissues, such as lymph nodes and spleen. CD14 is expressed at high levels, also on a few CD1c (BDCA-1)
+
CD2
+
myeloid dendritic cells and at low levels on neutrophilic granulocytes.
Ex vivo
differentiation of monocytes to dendritic cells is associated with down–regulation of CD14 antigen expression.

Alternative names

LPS R

Technical specifications

  • Antigen: CD14
  • Clone: TÜK4
  • Isotype: mouse IgG2aκ
  • Isotype control: Mouse IgG2a – isotype control antibodies
  • Alternative names of antigen: LPS R
  • Entrez Gene ID: 929
  • Molecular mass of antigen [kDa]: 37
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
    ,
    pigtail monkey (
    Macaca nemestrina
    )
    ,
    cotton-top tamarin (
    Saguinus oedipus
    )
    , pig, cow, sheep, goat, dog, mink, rabbit
  • Distribution of antigen: B cells, dendritic cells, epithelial cells, granulocytes, Langerhans cells, liver, macrophages, monocytes, osteoclasts, plasma cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, VioGreen, PerCP, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin
  • Selected references

    1. Jacobsen, C. N. et al. (1993) Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species. Vet. Immunol. Immunopathol. 39: 461-466
    2. Goyer, S. M. and Ferrero, E. (1987) Biochemical analysis of myeloid antigen and cDNA expression of gp55 (CD14). Oxford, Oxford University Press
    3. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
    4. Meissner, F. et al. (2010) Inflammasome activation in NADPH oxidase defective mononuclear phagocytes from patients with chronic granulomatous disease. Blood 116(9): 1570-1573
    5. Wilson, A. D. et al. (1995)
      Selection of monoclonal antibodies for the identification of lymphocyte surface antigens in the New World primate
      Saguinus oedipus oedipus
      (cotton top tamarin).
      J. Immunol. Methods 178: 195-200
    6. Neu, C. et al. (2013) CD14-dependent monocyte isolation enhances phagocytosis of listeria monocytogenes by proinflammatory, GM-CSF-derived macrophages. PLoS One 8(6): e66898
    7. Paccou, J. et al. (2013)
      Determination and modulation of total and surface calcium-sensing receptor expression in monocytes
      in vivo
      and
      in vitro
      .
      PLoS One 8(10): e74800
  • Customer reports and application notes

Product options: 22
130-113-708

CD14-FITC, human

for 30
tests
(1)
CAD 110.00
130-113-146

CD14-FITC, human

for 100
tests
(1)
CAD 330.00
130-113-709

CD14-PE, human

for 30
tests
(1)
CAD 140.00
130-113-147

CD14-PE, human

for 100
tests
(1)
CAD 380.00
130-113-705

CD14-APC, human

for 30
tests
(1)
CAD 165.00
130-113-143

CD14-APC, human

for 100
tests
(1)
CAD 390.00
for 30
tests
(1)
CAD 175.00
for 100
tests
(1)
CAD 430.00
for 30
tests
(1)
CAD 215.00
for 100
tests
(1)
CAD 470.00
for 30
tests
(1)
CAD 160.00
for 100
tests
(1)
CAD 440.00
for 30
tests
(1)
CAD 195.00
for 100
tests
(1)
CAD 415.00
for 30
tests
(1)
CAD 200.00
for 100
tests
(1)
CAD 435.00
for 30
tests
(1)
CAD 190.00
for 100
tests
(1)
CAD 440.00
for 30
tests
(1)
CAD 190.00
for 100
tests
(1)
CAD 440.00
for 30
tests
(1)
CAD 110.00
for 100
tests
(1)
CAD 330.00

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