Anti-TNF-α antibodies, human

Anti-TNF-α antibodies, human

Clone: cA2 | Dilution: 1:50
Tumor necrosis factor alpha (TNF-α) is produced by cells that are involved in inflammatory immune responses. TNF-α is secreted by activated CD4
+
T cells, monocytes, macrophages, NK cells, and neutrophils.
Anti-TNF-α antibodies have been designed for intracellular staining of TNF-α–producing cells. Cells can be stimulated for TNF-α production, for example, by polyclonal stimulation with mitogens. For induction of TNF-α production by antigen-specific T cells, cells are restimulated with the respective antigen. TNF-α can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, TNF-α–producing cells can be stained intracellularly with Anti-TNF-α antibodies. Staining for surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the TNF-α–producing cells.

Alternative names

TNF, DIF, TNFSF2

Technical specifications

  • Antigen: TNF-α
  • Clone: cA2
  • Isotype: human IgG1
  • Alternative names of antigen: TNF, DIF, TNFSF2
  • Entrez Gene ID: 7124
  • Molecular mass of antigen [kDa]: 26
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio770, APC-Vio770
Product options: 10
for 30
tests
(1)
CAD 105.00
for 100
tests
(1)
CAD 315.00
for 30
tests
(1)
CAD 135.00
for 100
tests
(1)
CAD 360.00
for 30
tests
(1)
CAD 160.00
for 100
tests
(1)
CAD 370.00
for 30
tests
(1)
CAD 190.00
for 100
tests
(1)
CAD 405.00
for 30
tests
(1)
CAD 175.00
for 100
tests
(1)
CAD 415.00

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