Anti-Ly-49I antibodies – functional grade, mouse

Anti-Ly-49I antibodies – functional grade, mouse

Clone: YLI-90
Clone YLI-90 recognizes the mouse Ly-49I antigen, a single-pass type II C-type lectin-like membrane glycoprotein also known as Ly-49 inhibitory receptor I. The Ly-49 receptors are encoded by a family of highly polymorphic and polygenic genes within the mouse NK gene complex which includes genes that encode both inhibitory and activating Ly-49 receptors. Ly-49 receptors recognize MHC-I and MHC-I-like proteins on normal as well as altered cells. They are described as being expressed on both the developing and mature NK cells. The inhibitory Ly-49 receptors are involved in NK cell education, a process in which NK cells acquire function and tolerance toward cells that express “self-MHC-I.” On the other hand, the activating Ly-49 receptors recognize altered cells expressing activating ligands. Ly-49 receptors have been described on multiple NK cell subsets, such as uterine NK and memory NK cells, as well as NKT cells, dendritic cells, plasmacytoid dendritic cells, macrophages, neutrophils, and cells of the adaptive immune system, such as activated T cells and regulatory CD8
+
T cells. Ly-49I is an inhibitory receptor capable of down-modulating proliferation, IFN-γ response, and cytotoxic activity in cells that express it.

Applications

This pure – functional grade antibody is suited for cell culture functional assays, for example, blocking of receptor-ligand binding.

Alternative names

Klra9, LY49I1

Technical specifications

  • Antigen: Ly-49I
  • Clone: YLI-90
  • Isotype: mouse IgG1κ
  • Alternative names of antigen: Klra9, LY49I1
  • Entrez Gene ID: 16640
  • Molecular mass of antigen [kDa]: 31
  • Distribution of antigen: NK cells
  • Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates:
  • Selected references

    1. Wong, S. et al. (1991) Ly-49 multigene family. New members of a superfamily of type II membrane proteins with lectin-like domains. J. Immunol. 147(4): 1417-1423
    2. Regunathan, J. et al. (2005) NKG2D receptor-mediated NK cell function is regulated by inhibitory Ly49 receptors. Blood 105: 233-240
    3. Tu, M. M. et al. (2014) Ly49 family receptors are required for cancer immunosurveillance mediated by natural killer cells. Cancer Res. 74(14): 3684-3694
    4. Dimasi, N. et al. (2002) Crystal structure of the Ly49I natural killer cell receptor reveals variability in dimerization mode within the Ly49 family. J. Mol. Biol. 320(3): 573-585
Product options: 1
500 µg in 0.5 mL
CAD 485.00

Related products

1 products available