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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Example of a separation using the REAlease® CD4 (TIL) MicroBead Kit.
A) A human ovarian carcinoma sample was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, human (# 130-095-929). CD4+
TILs were isolated from the single-cell suspension using the REAlease®
CD4 (TIL) MicroBead Kit, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with CD4-VioGreen™ and analyzed by flow cytometry using the MACSQuant®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD fluorescence. B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD4+
cells"), whereas the label-free CD4+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD4 (TIL)).
A) Cell purity
B) Label-free cells: REAlease Biotin Complex release