CD4+ CD25+ CD45RA+ Regulatory T Cell Isolation Kit, human

CD4
+
CD25
+
CD45RA
+
Regulatory T Cell Isolation Kit
, human

The CD4
+
CD25
+
CD45RA
+
Regulatory T Cell Isolation Kit was developed for the isolation of CD45RA
+
regulatory T cells (Treg) from human PBMCs in a two-step procedure.

Background information

Regulatory CD4
+
CD25
+
T cells isolated from the CD45RA
+
naive T cell compartment were shown to be optimal for
in vitro
expansion. These expanded Treg cells maintained the FoxP3
+
phenotype and their suppressive activity, whereas CD45RA
regulatory T cells from the memory T cell compartment lost FoxP3 expression and showed only modest suppressive activity.
1

Detailed separation procedure

The isolation is performed in a two-step procedure. First, non-CD4
+
and memory T cells are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. The labeled cells are subsequently depleted over a MACS
®
Column. In the second step, the flow-through fraction of pre-enriched CD4
+
CD45RA
+
T cells is labeled with CD25 MicroBeads for subsequent positive selection of CD4
+
CD25
+
CD45RA
+
T cells.

Downstream applications

CD4
+
CD25
+
CD45RA
+
regulatory T cells can be isolated from human PBMCs with this kit, for example, for further phenotypical or functional characterization and expansion.

Columns

For the first magnetic separation (depletion): LD or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.
  • Selected references

    1. Hoffmann, P. et al. (2006)
      Only the CD45RA
      +
      subpopulation of CD4
      +
      CD25
      high
      T cells gives rise to homogeneous regulatory T-cell lines upon
      in vitro
      expansion.
      Blood 108: 4260-4267
  • Brochures and posters

Product options: 1
for 2×
10
9
total cells
CAD 1,030.00

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