Find the products and resources you are looking for!
Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
T cells were enriched using the CD154 Enrichment and Detection Kit, two MS Columns, and a MiniMACS™ Separator. Spleen cells from a KLH immunized BALB/c mouse were restimulated in vitro with 100 μg/mL KLH or were not restimulated in the presence of the CD154 Detection Cocktail (PE) for 6 hours.
T cells were magnetically isolated using Anti-PE MicroBeads UltraPure. Cells were fluorescently stained with CD4-FITC and CD45R (B220)-PerCP. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence. CD45R+
cells were excluded from the analysis.