Human BMP-2

Human BMP-2

BMP-2 stands for bone morphogenetic protein 2. Human BMP-2 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human BMP-2 can be used for a variety of applications, including:
  • Study of chondrogenesis and osteogenesis.
  • Differentiation of mesenchymal stem cells into several cell type including osteoblast and cardiac cells.
  • Investigation of TGF-β signaling pathway.

Background information

Bone morphogenetic protein 2 (BMP-2) is a member of the bone morphogenetic protein family, which is part of the TGF-β superfamily. Together with BMP-7, BMP-2 is a potent stimulator of osteoblast differentiation as well as of chondrocyte proliferation of mesenchymal cells during bone and cartilage formation, and can also induce cardiac and epithelial morphogenesis. BMP-2 is secreted by several cell tissues such as spleen, lung, liver, brain, small intestine, prostate, and ovary, as immature long form, and after proteolytic cleavage it matures into an active disulfide-linked homodimer. BMP-2 signals through heterodimeric receptors, such as BMPR1A, and activates TGF-β signaling cascade and the hedgehog pathway. Besides the morphogenic role, BMP-2 is also crucial during embryonic dorsal-ventral generation and organogenesis. Recombinant BMP-2 is under clinical investigation for treatment of bone degeneration diseases, spinal injuries, and orthodontic pathologies. Mature human mouse and rat BMP-2 share full amino acid sequence identity. Recombinant Human BMP-2 corresponds to the mature form of BMP-2.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

  • Alkaline phosphatase assay using mouse C2C12 fibroblasts (NIBSC 93/574)
  • premium grade: ≥ 1×
    10
    5
    U/mg
  • research grade: ≥ 5×
    10
    4
    U/mg
  • Selected references

    1. Cheng, H. et al. (2003) Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs). J Bone Joint Surg Am 85(A8): 1544-1552
Product options: 6
E. coli
10 µg
CAD 260.00
E. coli
25 µg
CAD 485.00
E. coli
10 µg
CAD 320.00
E. coli
25 µg
CAD 620.00
E. coli
100 µg
CAD 1,450.00
E. coli
1000 µg
CAD 5,660.00