Clone:
REA441
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
ITGB7, Ly69, M290 IEL

Extended validation for Integrin β7 Antibody, anti-human/mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA441
FIB504++
473207-
Cells were incubated with an excess of purified unconjugated Integrin β7 (REA441) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Integrin β7. Human peripheral blood mononuclear cells (PBMCs) were stained with Integrin β7 antibodies and with a suitable counterstaining. As a control, Integrin β7 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Integrin β7. Human peripheral blood mononuclear cells (PBMCs) were stained with Integrin β7 antibodies and with a suitable counterstaining. As a control, Integrin β7 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Integrin β7. Human peripheral blood mononuclear cells (PBMCs) were stained with Integrin β7 antibodies and with a suitable counterstaining. As a control, Integrin β7 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Integrin β7. Human peripheral blood mononuclear cells (PBMCs) were stained with Integrin β7 antibodies and with a suitable counterstaining. As a control, Integrin β7 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Integrin β7. Human peripheral blood mononuclear cells (PBMCs) were stained with Integrin β7 antibodies and with a suitable counterstaining. As a control, Integrin β7 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using Integrin β7 (REA441). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using Integrin β7 (REA441). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using Integrin β7 (REA441). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for Integrin β7 Antibody, anti-human/mouse, REAfinity™

Overview

Clone REA441 recognizes the human and mouse integrin β7 antigen, a single-pass type I membrane protein which is also known as gut homing receptor β subunit. Integrins are heterodimeric glycoprotein receptors and exist as non-covalently bound α and β subunits. The integrin β7 subfamily has two known members (α4β7 and αEβ7) that are expressed primarily by leukocytes and have been implicated in secondary lymphoid structure formation, plasma cells homing to gastrointestinal mucosa, and inflammatory responses. Their ligands: the mucosal vascular addressin cell adhesion molecule 1 (MAdCAM-1) and E-cadherin are present on the surface of endothelial cells, bone marrow stromal cells, and epithelial cells. Interaction of α4β7 with MAdCAM-1 allows for tissue-specific migration of circulating lymphocytes into the lamina propria and Peyer patches of the gut, whereas αEβ7 retains intraepithelial lymphocytes within the gut epithelium through E-cadherin binding. Integrin β7 is also reported to be involved in the pathogenesis of several diseases such as colitis, diabetic insulitis, and lymphoid malignancies including lymphomatous polyposis in mantle cell lymphoma, thymic lymphoma, and mucosa-associated T cell and B cell non-Hodgkin lymphomas.
Additional information: Clone REA441 displays negligible binding to Fc receptors.

Alternative names

ITGB7, Ly69, M290 IEL

Detailed product information

Technical specifications

CloneREA441
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse
AntigenIntegrin β7
Alternative names of antigenITGB7, Ly69, M290 IEL
Molecular mass of antigen [kDa]86
Distribution of antigenleukocytes
Entrez Gene ID16421
RRIDAB_2751540, AB_2752017, AB_2751987, AB_2819501, AB_2819493, AB_2801890, AB_2652508, AB_2652509, AB_2922250, AB_2922216, AB_2922294, AB_2922288, AB_2751550

Resources for Integrin β7 Antibody, anti-human/mouse, REAfinity™

Certificates

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References for Integrin β7 Antibody, anti-human/mouse, REAfinity™

Publications

  1. Alles, M. et al. (2014) Leukocyte β7 integrin targeted by Krüppel-like factors. J. Immunol. 193(4): 1737-1746
  2. Neri, P. et al. (2011) Integrin β7-mediated regulation of multiple myeloma cell adhesion, migration, and invasion. Blood 117(23): 6202-6213
  3. Hu, M. C. et al. (1992) Cloning and expression of mouse integrin beta p(beta 7): a functional role in Peyer's patch-specific lymphocyte homing. Proc. Natl. Acad. Sci. U.S.A. 89(17): 8254-8258

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