Cookie Settings
We use cookies to provide the best possible website experience for you. This includes cookies that are technically required to ensure a proper functioning of the website, as well as cookies which are used solely for anonymous statistical purposes, for more comfortable website settings, or for displaying personalized content. You are free to choose the categories you would like to permit. Please note that depending on your settings, the full functionality of the website may no longer be available. Further information can be found in our Privacy Statement and Cookie Statement.
Type 2 innate lymphoid cells (ILC2) were isolated from peripheral blood mononuclear cells by using the ILC2 Isolation Kit, two LS and two MS Columns, as well as a MidiMACS ™ Separator and a MiniMACS ™ Separator. The cells were fluorescently stained with CD45-VioBlue ® and lineage markers (CD2, CD3, CD14, CD16, CD123, CD235a (Glycophorin A) conjugated to FITC, CD19 and CD56 conjugated to VioBright ™ FITC), and CD294 (CRTH2)-PE (A) or CD161-PE-Vio ® 770 and CD127-APC (B) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from analysis based on scatter signals, CD45, and propidium iodide fluorescence. | |
A: | B: |
Before separation | |
 View details ILC2 Isolation Kit, humanFigure 1Type 2 innate lymphoid cells (ILC2) were isolated from peripheral blood mononuclear cells by using the ILC2 Isolation Kit, two LS and two MS Columns, as well as a MidiMACS ™ Separator and a MiniMACS ™ Separator. The cells were fluorescently stained with CD45-VioBlue ® and lineage markers (CD2, CD3, CD14, CD16, CD123, CD235a (Glycophorin A) conjugated to FITC, CD19 and CD56 conjugated to VioBright ™ FITC), and CD294 (CRTH2)-PE (A) or CD161-PE-Vio ® 770 and CD127-APC (B) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from analysis based on scatter signals, CD45, and propidium iodide fluorescence. |  View details ILC2 Isolation Kit, humanFigure 1Type 2 innate lymphoid cells (ILC2) were isolated from peripheral blood mononuclear cells by using the ILC2 Isolation Kit, two LS and two MS Columns, as well as a MidiMACS ™ Separator and a MiniMACS ™ Separator. The cells were fluorescently stained with CD45-VioBlue ® and lineage markers (CD2, CD3, CD14, CD16, CD123, CD235a (Glycophorin A) conjugated to FITC, CD19 and CD56 conjugated to VioBright ™ FITC), and CD294 (CRTH2)-PE (A) or CD161-PE-Vio ® 770 and CD127-APC (B) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from analysis based on scatter signals, CD45, and propidium iodide fluorescence. |
Lineage-negative cells | |
 View details ILC2 Isolation Kit, humanFigure 1Type 2 innate lymphoid cells (ILC2) were isolated from peripheral blood mononuclear cells by using the ILC2 Isolation Kit, two LS and two MS Columns, as well as a MidiMACS ™ Separator and a MiniMACS ™ Separator. The cells were fluorescently stained with CD45-VioBlue ® and lineage markers (CD2, CD3, CD14, CD16, CD123, CD235a (Glycophorin A) conjugated to FITC, CD19 and CD56 conjugated to VioBright ™ FITC), and CD294 (CRTH2)-PE (A) or CD161-PE-Vio ® 770 and CD127-APC (B) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from analysis based on scatter signals, CD45, and propidium iodide fluorescence. |  View details ILC2 Isolation Kit, humanFigure 1Type 2 innate lymphoid cells (ILC2) were isolated from peripheral blood mononuclear cells by using the ILC2 Isolation Kit, two LS and two MS Columns, as well as a MidiMACS ™ Separator and a MiniMACS ™ Separator. The cells were fluorescently stained with CD45-VioBlue ® and lineage markers (CD2, CD3, CD14, CD16, CD123, CD235a (Glycophorin A) conjugated to FITC, CD19 and CD56 conjugated to VioBright ™ FITC), and CD294 (CRTH2)-PE (A) or CD161-PE-Vio ® 770 and CD127-APC (B) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from analysis based on scatter signals, CD45, and propidium iodide fluorescence. |
Isolated CD294 (CRTH2)+ ILC2 | |
 View details ILC2 Isolation Kit, humanFigure 1Type 2 innate lymphoid cells (ILC2) were isolated from peripheral blood mononuclear cells by using the ILC2 Isolation Kit, two LS and two MS Columns, as well as a MidiMACS ™ Separator and a MiniMACS ™ Separator. The cells were fluorescently stained with CD45-VioBlue ® and lineage markers (CD2, CD3, CD14, CD16, CD123, CD235a (Glycophorin A) conjugated to FITC, CD19 and CD56 conjugated to VioBright ™ FITC), and CD294 (CRTH2)-PE (A) or CD161-PE-Vio ® 770 and CD127-APC (B) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from analysis based on scatter signals, CD45, and propidium iodide fluorescence. |  View details ILC2 Isolation Kit, humanFigure 1Type 2 innate lymphoid cells (ILC2) were isolated from peripheral blood mononuclear cells by using the ILC2 Isolation Kit, two LS and two MS Columns, as well as a MidiMACS ™ Separator and a MiniMACS ™ Separator. The cells were fluorescently stained with CD45-VioBlue ® and lineage markers (CD2, CD3, CD14, CD16, CD123, CD235a (Glycophorin A) conjugated to FITC, CD19 and CD56 conjugated to VioBright ™ FITC), and CD294 (CRTH2)-PE (A) or CD161-PE-Vio ® 770 and CD127-APC (B) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from analysis based on scatter signals, CD45, and propidium iodide fluorescence. |
Type 2 innate lymphoid cells (ILC2) were isolated from peripheral blood mononuclear cells by using the ILC2 Isolation Kit, two LS and two MS Columns, as well as a MidiMACS ™ Separator and a MiniMACS ™ Separator. The cells were fluorescently stained with CD45-VioBlue ® and lineage markers (CD2, CD3, CD14, CD16, CD123, CD235a (Glycophorin A) conjugated to FITC, CD19 and CD56 conjugated to VioBright ™ FITC), and CD294 (CRTH2)-PE (A) or CD161-PE-Vio ® 770 and CD127-APC (B) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from analysis based on scatter signals, CD45, and propidium iodide fluorescence. | |
A: | B: |
Before separation | |
View details ILC2 Isolation Kit, humanFigure 1Type 2 innate lymphoid cells (ILC2) were isolated from peripheral blood mononuclear cells by using the ILC2 Isolation Kit, two LS and two MS Columns, as well as a MidiMACS ™ Separator and a MiniMACS ™ Separator. The cells were fluorescently stained with CD45-VioBlue ® and lineage markers (CD2, CD3, CD14, CD16, CD123, CD235a (Glycophorin A) conjugated to FITC, CD19 and CD56 conjugated to VioBright ™ FITC), and CD294 (CRTH2)-PE (A) or CD161-PE-Vio ® 770 and CD127-APC (B) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from analysis based on scatter signals, CD45, and propidium iodide fluorescence. | View details ILC2 Isolation Kit, humanFigure 1Type 2 innate lymphoid cells (ILC2) were isolated from peripheral blood mononuclear cells by using the ILC2 Isolation Kit, two LS and two MS Columns, as well as a MidiMACS ™ Separator and a MiniMACS ™ Separator. The cells were fluorescently stained with CD45-VioBlue ® and lineage markers (CD2, CD3, CD14, CD16, CD123, CD235a (Glycophorin A) conjugated to FITC, CD19 and CD56 conjugated to VioBright ™ FITC), and CD294 (CRTH2)-PE (A) or CD161-PE-Vio ® 770 and CD127-APC (B) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from analysis based on scatter signals, CD45, and propidium iodide fluorescence. |
Lineage-negative cells | |
View details ILC2 Isolation Kit, humanFigure 1Type 2 innate lymphoid cells (ILC2) were isolated from peripheral blood mononuclear cells by using the ILC2 Isolation Kit, two LS and two MS Columns, as well as a MidiMACS ™ Separator and a MiniMACS ™ Separator. The cells were fluorescently stained with CD45-VioBlue ® and lineage markers (CD2, CD3, CD14, CD16, CD123, CD235a (Glycophorin A) conjugated to FITC, CD19 and CD56 conjugated to VioBright ™ FITC), and CD294 (CRTH2)-PE (A) or CD161-PE-Vio ® 770 and CD127-APC (B) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from analysis based on scatter signals, CD45, and propidium iodide fluorescence. | View details ILC2 Isolation Kit, humanFigure 1Type 2 innate lymphoid cells (ILC2) were isolated from peripheral blood mononuclear cells by using the ILC2 Isolation Kit, two LS and two MS Columns, as well as a MidiMACS ™ Separator and a MiniMACS ™ Separator. The cells were fluorescently stained with CD45-VioBlue ® and lineage markers (CD2, CD3, CD14, CD16, CD123, CD235a (Glycophorin A) conjugated to FITC, CD19 and CD56 conjugated to VioBright ™ FITC), and CD294 (CRTH2)-PE (A) or CD161-PE-Vio ® 770 and CD127-APC (B) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from analysis based on scatter signals, CD45, and propidium iodide fluorescence. |
Isolated CD294 (CRTH2)+ ILC2 | |
View details ILC2 Isolation Kit, humanFigure 1Type 2 innate lymphoid cells (ILC2) were isolated from peripheral blood mononuclear cells by using the ILC2 Isolation Kit, two LS and two MS Columns, as well as a MidiMACS ™ Separator and a MiniMACS ™ Separator. The cells were fluorescently stained with CD45-VioBlue ® and lineage markers (CD2, CD3, CD14, CD16, CD123, CD235a (Glycophorin A) conjugated to FITC, CD19 and CD56 conjugated to VioBright ™ FITC), and CD294 (CRTH2)-PE (A) or CD161-PE-Vio ® 770 and CD127-APC (B) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from analysis based on scatter signals, CD45, and propidium iodide fluorescence. | View details ILC2 Isolation Kit, humanFigure 1Type 2 innate lymphoid cells (ILC2) were isolated from peripheral blood mononuclear cells by using the ILC2 Isolation Kit, two LS and two MS Columns, as well as a MidiMACS ™ Separator and a MiniMACS ™ Separator. The cells were fluorescently stained with CD45-VioBlue ® and lineage markers (CD2, CD3, CD14, CD16, CD123, CD235a (Glycophorin A) conjugated to FITC, CD19 and CD56 conjugated to VioBright ™ FITC), and CD294 (CRTH2)-PE (A) or CD161-PE-Vio ® 770 and CD127-APC (B) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from analysis based on scatter signals, CD45, and propidium iodide fluorescence. |
Seems like you are coming from USA!
Do you want to visit our website in your country?
Copyright © 2023 Miltenyi Biotec and/or its affiliates. All rights reserved.
