Cookie Settings
We use cookies to provide the best possible website experience for you. This includes cookies that are technically required to ensure a proper functioning of the website, as well as cookies which are used solely for anonymous statistical purposes, for more comfortable website settings, or for displaying personalized content. You are free to choose the categories you would like to permit. Please note that depending on your settings, the full functionality of the website may no longer be available. Further information can be found in our Privacy Statement and Cookie Statement.
Human peripheral blood mononuclear cells (PBMCs) were incubated with or without (left images) LPS for six hours. After two hours, brefeldin A was added. Cells were harvested, then stained with CD14 antibodies, fixed, permeabilized, and intracellularly stained with Anti-IL-6 antibodies. The FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. Cells were analyzed by flow cytometry using the MACSQuant® Analyzer. Gating was performed on monocytes according to scatter properties of the cells. Autofluorescent cell debris was excluded from the analysis in a fluorescence 4 (FL-4/B3) versus fluorescence 3 (FL-3/B2) dot plot.
Other clones | Overlap in epitope recognition with MQ2-13A5 |
---|---|
REA1037 | ++ |
AS12 | - |
MQ2-6A3 | - |
Cells were incubated with an excess of purified unconjugated IL-6 (MQ2-13A5) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker. |
Clone | MQ2-13A5 |
---|---|
Clonality | monoclonal |
Isotype | rat IgG1, rat IgG1κ |
Isotype control | Isotype Control Antibody, rat IgG1 |
Host | rat |
Type of antibody | Primary antibodies, Functional-grade antibodies |
Species | human |
Antigen | IL-6 |
Alternative names of antigen | BSF2, HGF, HSF, IFNB2 |
Molecular mass of antigen [kDa] | 21 |
Entrez Gene ID | 3569 |
RRID | AB_2652449, AB_2652450, AB_2652451, AB_2652452, AB_2652453, AB_2652454 |
Other clones | Overlap in epitope recognition with MQ2-13A5 |
---|---|
REA1037 | ++ |
AS12 | - |
MQ2-6A3 | - |
Cells were incubated with an excess of purified unconjugated IL-6 (MQ2-13A5) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker. |
Clone | MQ2-13A5 |
---|---|
Clonality | monoclonal |
Isotype | rat IgG1, rat IgG1κ |
Isotype control | Isotype Control Antibody, rat IgG1 |
Host | rat |
Type of antibody | Primary antibodies, Functional-grade antibodies |
Species | human |
Antigen | IL-6 |
Alternative names of antigen | BSF2, HGF, HSF, IFNB2 |
Molecular mass of antigen [kDa] | 21 |
Entrez Gene ID | 3569 |
RRID | AB_2652449, AB_2652450, AB_2652451, AB_2652452, AB_2652453, AB_2652454 |
Seems like you are coming from USA!
Do you want to visit our website in your country?
Copyright © 2023 Miltenyi Biotec and/or its affiliates. All rights reserved.