Adult Neural Stem Cell Analysis Cocktail Kit, anti-mouse

Name: Adult Neural Stem Cell Analysis Cocktail Kit, anti-mouse
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Adult Neural Stem Cell Analysis Cocktail Kit, anti-mouse

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Product data and images for Adult Neural Stem Cell Analysis Cocktail Kit, anti-mouse

Figures

Figure 1

Dissociation of subventricular zone tissue yielded 3.69×10

⁵

± 9.3×10

⁴

cells per mouse (7–9 weeks old) with a viability rate of >97% (n = 12), using Neural Tissue Dissociation Kit (T) and gentleMACS™ Octo with Heaters.

This figure shows a representative analysis of the different fractions after exclusion of debris, doublets, and dead cells. Sorting of 3.69×10

⁵

± 9.3×10

⁴

total cells resulted in 36,000 ± 8,000 GLAST

⁺

Plexin-B2

⁺

neural stem cells with a purity of >95% and a viability rate of >93% as analyzed by propidium iodide staining (n = 6).

Non-sorted cells	Positive fraction	Negative fraction
View details Adult Neural Stem Cell Analysis Cocktail Kit, anti-mouse Figure 1 Dissociation of subventricular zone tissue yielded 3.69×10 ⁵ ± 9.3×10 ⁴ cells per mouse (7–9 weeks old) with a viability rate of >97% (n = 12), using Neural Tissue Dissociation Kit (T) and gentleMACS™ Octo with Heaters. This figure shows a representative analysis of the different fractions after exclusion of debris, doublets, and dead cells. Sorting of 3.69×10 ⁵ ± 9.3×10 ⁴ total cells resulted in 36,000 ± 8,000 GLAST ⁺ Plexin-B2 ⁺ neural stem cells with a purity of >95% and a viability rate of >93% as analyzed by propidium iodide staining (n = 6).	View details Adult Neural Stem Cell Analysis Cocktail Kit, anti-mouse Figure 1 Dissociation of subventricular zone tissue yielded 3.69×10 ⁵ ± 9.3×10 ⁴ cells per mouse (7–9 weeks old) with a viability rate of >97% (n = 12), using Neural Tissue Dissociation Kit (T) and gentleMACS™ Octo with Heaters. This figure shows a representative analysis of the different fractions after exclusion of debris, doublets, and dead cells. Sorting of 3.69×10 ⁵ ± 9.3×10 ⁴ total cells resulted in 36,000 ± 8,000 GLAST ⁺ Plexin-B2 ⁺ neural stem cells with a purity of >95% and a viability rate of >93% as analyzed by propidium iodide staining (n = 6).	View details Adult Neural Stem Cell Analysis Cocktail Kit, anti-mouse Figure 1 Dissociation of subventricular zone tissue yielded 3.69×10 ⁵ ± 9.3×10 ⁴ cells per mouse (7–9 weeks old) with a viability rate of >97% (n = 12), using Neural Tissue Dissociation Kit (T) and gentleMACS™ Octo with Heaters. This figure shows a representative analysis of the different fractions after exclusion of debris, doublets, and dead cells. Sorting of 3.69×10 ⁵ ± 9.3×10 ⁴ total cells resulted in 36,000 ± 8,000 GLAST ⁺ Plexin-B2 ⁺ neural stem cells with a purity of >95% and a viability rate of >93% as analyzed by propidium iodide staining (n = 6).
View details Adult Neural Stem Cell Analysis Cocktail Kit, anti-mouse Figure 1 Dissociation of subventricular zone tissue yielded 3.69×10 ⁵ ± 9.3×10 ⁴ cells per mouse (7–9 weeks old) with a viability rate of >97% (n = 12), using Neural Tissue Dissociation Kit (T) and gentleMACS™ Octo with Heaters. This figure shows a representative analysis of the different fractions after exclusion of debris, doublets, and dead cells. Sorting of 3.69×10 ⁵ ± 9.3×10 ⁴ total cells resulted in 36,000 ± 8,000 GLAST ⁺ Plexin-B2 ⁺ neural stem cells with a purity of >95% and a viability rate of >93% as analyzed by propidium iodide staining (n = 6).	View details Adult Neural Stem Cell Analysis Cocktail Kit, anti-mouse Figure 1 Dissociation of subventricular zone tissue yielded 3.69×10 ⁵ ± 9.3×10 ⁴ cells per mouse (7–9 weeks old) with a viability rate of >97% (n = 12), using Neural Tissue Dissociation Kit (T) and gentleMACS™ Octo with Heaters. This figure shows a representative analysis of the different fractions after exclusion of debris, doublets, and dead cells. Sorting of 3.69×10 ⁵ ± 9.3×10 ⁴ total cells resulted in 36,000 ± 8,000 GLAST ⁺ Plexin-B2 ⁺ neural stem cells with a purity of >95% and a viability rate of >93% as analyzed by propidium iodide staining (n = 6).	View details Adult Neural Stem Cell Analysis Cocktail Kit, anti-mouse Figure 1 Dissociation of subventricular zone tissue yielded 3.69×10 ⁵ ± 9.3×10 ⁴ cells per mouse (7–9 weeks old) with a viability rate of >97% (n = 12), using Neural Tissue Dissociation Kit (T) and gentleMACS™ Octo with Heaters. This figure shows a representative analysis of the different fractions after exclusion of debris, doublets, and dead cells. Sorting of 3.69×10 ⁵ ± 9.3×10 ⁴ total cells resulted in 36,000 ± 8,000 GLAST ⁺ Plexin-B2 ⁺ neural stem cells with a purity of >95% and a viability rate of >93% as analyzed by propidium iodide staining (n = 6).
View details Adult Neural Stem Cell Analysis Cocktail Kit, anti-mouse Figure 1 Dissociation of subventricular zone tissue yielded 3.69×10 ⁵ ± 9.3×10 ⁴ cells per mouse (7–9 weeks old) with a viability rate of >97% (n = 12), using Neural Tissue Dissociation Kit (T) and gentleMACS™ Octo with Heaters. This figure shows a representative analysis of the different fractions after exclusion of debris, doublets, and dead cells. Sorting of 3.69×10 ⁵ ± 9.3×10 ⁴ total cells resulted in 36,000 ± 8,000 GLAST ⁺ Plexin-B2 ⁺ neural stem cells with a purity of >95% and a viability rate of >93% as analyzed by propidium iodide staining (n = 6).	View details Adult Neural Stem Cell Analysis Cocktail Kit, anti-mouse Figure 1 Dissociation of subventricular zone tissue yielded 3.69×10 ⁵ ± 9.3×10 ⁴ cells per mouse (7–9 weeks old) with a viability rate of >97% (n = 12), using Neural Tissue Dissociation Kit (T) and gentleMACS™ Octo with Heaters. This figure shows a representative analysis of the different fractions after exclusion of debris, doublets, and dead cells. Sorting of 3.69×10 ⁵ ± 9.3×10 ⁴ total cells resulted in 36,000 ± 8,000 GLAST ⁺ Plexin-B2 ⁺ neural stem cells with a purity of >95% and a viability rate of >93% as analyzed by propidium iodide staining (n = 6).	View details Adult Neural Stem Cell Analysis Cocktail Kit, anti-mouse Figure 1 Dissociation of subventricular zone tissue yielded 3.69×10 ⁵ ± 9.3×10 ⁴ cells per mouse (7–9 weeks old) with a viability rate of >97% (n = 12), using Neural Tissue Dissociation Kit (T) and gentleMACS™ Octo with Heaters. This figure shows a representative analysis of the different fractions after exclusion of debris, doublets, and dead cells. Sorting of 3.69×10 ⁵ ± 9.3×10 ⁴ total cells resulted in 36,000 ± 8,000 GLAST ⁺ Plexin-B2 ⁺ neural stem cells with a purity of >95% and a viability rate of >93% as analyzed by propidium iodide staining (n = 6).

Figure 2

Cultivation of isolated NSCs under the specified assay conditions led to formation of a large number of neurospheres, which gave rise to secondary neurospheres (A, B).Neurospheres differentiated into glial cells as well as neurons as shown by expression of GFAP, Nestin, GLAST, MAP2, and O4 (C–F).

Figure 2

Cultivation of isolated NSCs under the specified assay conditions led to formation of a large number of neurospheres, which gave rise to secondary neurospheres (A, B).Neurospheres differentiated into glial cells as well as neurons as shown by expression of GFAP, Nestin, GLAST, MAP2, and O4 (C–F).

Specifications for Adult Neural Stem Cell Analysis Cocktail Kit, anti-mouse

Overview

The Adult Neural Stem Cell Analysis Cocktail Kit, anti-mouse enables a reliable identification of neural stem cells (NSCs) from the subventricular zone (SVZ) of mouse brain tissue for subsequent sorting and analysis. The ready-to-use cocktail includes NSC-specific antibodies (GLAST and Plexin-B2 antibodies) and a set of surface antibodies to exclude erythrocytes, leukocytes, microglia, neurons, ependymal cells, and neuroblasts. A workflow protocol is established for fast and pure isolation of NSCs (total process time 2h, purity >95%, viability >93%).

Detailed product information

Background information

Adult Neural Stem Cell Analysis Cocktail, anti-mouse containing:

GLAST (ACSA-1) Antibody, anti-human/mouse/rat, APC (clone: ACSA-1)
Plexin-B2 Antibody, anti-mouse, PE, REAfinity™ (clone: REA445)
CD24 Antibody, anti-mouse, VioBlue®, REAfinity™ (clone: REA743)
CD45 Antibody, anti-mouse, VioBlue, REAfinity™ (clone: REA737)
Ter-119 Antibody, anti-mouse, VioBlue, REAfinity™ (clone: REA847)

Additionally

FcR Blocking Reagent, mouse

Instrument details

Flow cytometer equipped with a red (640 nm), a blue (488 nm), and a violet (405 nm) laser.

Applications

Identification and enumeration of NSCs from adult mouse SVZ for subsequent sorting and analysis.

Resources for Adult Neural Stem Cell Analysis Cocktail Kit, anti-mouse

Documents and Protocols

Scientific posters

A novel approach for efficient dissociation of distinct adult mouse brain regions and subsequent sorting of neurons and neural stem cells using MACSQuant® Tyto®

Scientific posters

A novel approach for gentle sorting of adult neural stem cells from wild type mouse brain using MACSQuant® Tyto®

App notes/customer reports

Sorting and cultivation of adult neural stem cells from mice

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

Data and images