7-AAD Staining Solution is a ready-to-use reagent suitable for the evaluation of cell viability in mono- or multiparametric analyses of human peripheral blood using flow cytometry.
7-AAD (7-amino-actinomycin D) is a fluorescent dye that intercalates into double-stranded DNA (GC rich regions). It is excluded from viable cells, but can penetrate cell membranes of dead or dying cells. Therefore, it can be used instead of propidium iodide (PI) for the evaluation of cell death and apoptosis.
The fluorescence emission maximum for 7-AAD is at 647 nm. When excited at 488 nm, 7-AAD is detected in the red fluorescence channel commonly used for