Enrichment and manufacturing of dendritic cells

  • Clinical-grade enrichment of monocytes and blood dendritic cells
  • Ex vivo GMP-compliant antigen loading of DCs
  • CliniMACS® System in combination with the MACS® GMP Products
Dendritic cells (DCs) are the most professional antigen-presenting cells and play a central role in eliciting specific adaptive and humoral immune responses. These features make DCs a very attractive tool for immunotherapeutic approaches.

Clinical scale Mo-DC manufacturing and electroporation

The preparation of cellular monocyte-derived dendritic cell (Mo-DC) products for clinical settings comprises several independent processing steps including density gradient centrifugation, monocyte separation, cell culture (differentiation and maturation), and possibly electroporation. To meet the increasing regulatory requirements for cell-based therapeutics, we have integrated all manufacturing steps in a closed system operated by an automated cell processing instrument, the CliniMACS Prodigy® with CliniMACS® Electroporator.

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Scientific poster
Monocyte-derived dendritic cells (Mo-DCs) generated with CliniMACS Prodigy® are functional and fulfill requirements for cancer vaccines

Mareke Brüning, Tobias Ozimkowski, Eva Bergschneider, Michael Birth, Stefan Wild, and Andrzej Dzionek, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany 


 

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Brochure
Manufacturing dendritic cell products for clinical research

This comprehensive brochure provides information regarding next generation clinical-grade production of DCs that may allow optimization of cellular products, formulation, and efficacy.

Related PDFs:

CliniMACS® CD14 System (reference list)

Automated enrichment and culture of blood DCs

Recent reports suggest that simultaneous activation of different DC subsets may have a synergistic effect that can increase anti-tumor responses. We have established the idea of a new clinical approach based on primary DCs derived from peripheral blood using the CliniMACS Prodigy System. The CliniMACS Prodigy CD1c/CD304 Enrichment and Activation System enables fully automated production of BDC-based vaccines with high purities and recoveries.


Related products:

MACS® GMP Reagents for ex-vivo GMP-compliant generation of DC vaccines

The CliniMACS® System in combination with our MACS® GMP Product Line offers a comprehensive portfolio optimized for the ex vivo GMP-compliant generation of DC vaccines.

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