MBTP 15: Immunophenotyping of neutrophils and eosinophils from mouse spleen using flow cytometry

Miltenyi Biotec-tested panel 15 (MBTP 15)

This application protocol describes the flow cytometric analysis of neutrophils and eosinophils after spleen dissociation from healthy C57BL/6 mice. Mouse spleens are easily dissociated using the gentleMACS™ Dissociators to quickly obtain viable single cell suspensions ready for flow cytometric analysis.

Protocol

Materials

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