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Challenges in NK cell immunotherapy: where do we stand?

This webinar will review the current status of NK cell-based immunotherapy. Martha Elia Luevano Salinas, product manager NK cells, will discuss different strategies used to generate or isolate NK cells for immunotherapy and current approaches boosting NK cell cytotoxicity. Please request the presentation or watch the webinar recording (October 2016).

Generation of highly enriched hiPSC-CMs for high-throughput cardiotoxicity screens

Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) are emerging as an ideal in vitro platform for pre-clinical cardiotoxicity screening of new chemical entities. In this webinar, Todd J. Herron, PhD, Director of the Cardiovascular Regeneration Core Laboratory, University of Michigan, shows how they utilized a non-genetic approach to generate highly enriched hiPSC-CMs using magnetic cell sorting to deplete non-cardiomyocytes from the cell population. Watch the recording or request the presentation of this webinar. (June 2017)

Clinical scale cultivation of MSCs – Tools to support an GMP-compliant workflow

The demand for xeno-free and GMP-compliant production of clinical-scale mesenchymal stem cells (MSCs) as well as standardized quality control measures for cellular products is rising. Kathrin Godthardt, Senior Scientist, R&D Department, Miltenyi Biotec, shows xeno-free MSC expansion solutions and workflows for qualifying the quality and phenotype of culture expanded MSCs. Furthermore, the CliniMACS Prodigy® will be introduced as an automated closed system for GMP-conform isolation and expansion of stem cells. Watch the recording or request the presentation of this webinar. (November 2016)

Novel tools for cardiac cell isolation

Neonatal cardiomyocytes (CMs) as well as cardiomyocytes derived from pluripotent stem cells (PSC) are commonly used to study biochemical, physiological, pharmacological, or morphological characteristics or investigate biochemical pathways. Gregor von Levetzow introduces in this webinar novel tools for cardiovascular research. Watch the webinar recording or request the presentation and see how easy primary CMs can be highly enriched from neonatal hearts or differentiated PSC cultures. (May 2016)

Foundations of stem cell maintenance

In this webinar, we’ll guide you through the different opportunities to culture cells and test their quality. Learn more about cell splitting techniques and find out how to sort pluripotent stem cells for continuous, high-level quality. Request the presentation or watch the webinar. (April 2016)

Foundations for stem cell reprogramming

Foundations for stem cell reprogramming is the first in a series of webinars on stem cell research. The 45-minute webinar introduces a highly efficient method for reprogramming into iPS cells and will deepen your stem cell knowledge. Request the presentation or watch the webinar recording. (August 2015)

Quantitative and qualitative analysis of ES and iPS cell culture by flow cytometry

Stem cells express a number of proteins that are considered to be markers of pluripotency. The differentiation of stem cells or the reprogramming of cells to iPS cells leads to changes in these markers. The – qualitative and quantitative – condition of a stem cell population can therefore be tracked using fluorescently tagged antibodies against these markers and flow cytometry. Sebastian Knöbel, PhD, Senior Project Manager Miltenyi Biotec R&D, shows examples of cell culture monitoring by flow cytometry and explains the critical factors to be considered for a valid analysis. Request the presentation of this webinar or watch the recording. (November 2013)

Isolation of carcinoma cells from FFPE samples for high-quality sequencing results
In this webinar, Bastian Nicolai introduces an innovative method for dissociation of FFPE carcinoma samples into single cell suspensions for high-quality sequencing results. See how the new FFPE Tissue Dissociation Kit from Miltenyi Biotec, also suited for any other tissue from any species, preserves cytoskeletal markers and improves molecular analysis. Dissociated cells can be subsequently separated by flow sorting or analyzed by flow cytometry in vitro for phenotype distributions, and other genetic or proteomic studies. Watch the webinar recording or request the presentation (pdf). (June 2018)

Isolation of tumor cells and TILs for optimized downstream analysis

For the development of novel immune-based therapeutic strategies, it is essential to study the interaction and phenotypes of immune and tumor cells within the tumor microenvironment in detail. In this webinar, Dr. Olaf Hardt introduces a sophisticated workflow that allows for the isolation of tumor cell subpopulations and tumor-infiltrating leukocytes (TILs). You will learn about optimized techniques for the dissociation of tumor tissue as well as the analysis of tumor cells and tumor-infiltrating immune cell subpopulations by flow cytometry. Watch the webinar recording or request the presentation (pdf). (May 2018)

Tumor microenvironment - Tools for TIL isolation and flow cytometry analysis
A deep understanding of the interactions and phenotypes of immune and tumor cells within the tumor microenvironment is key to developing future immune-based cancer therapy strategies. In this webinar, Dr. César Evaristo describes best practices for isolation of tumor infiltrating leukocytes (TILs) and present an optimized workflow for faster and improved flow analysis. You will be introduced to innovative tools and solutions to increase quality of data, reduce experimental time, and ease scale-up during TIL analysis using flow cytometry. Watch the webinar recording or request the presentation (pdf). (May 2018)

The MACSQuant® Analyzer 16 – Colorful new possibilities in automated flow cytometry 

As research questions become more complex, let the MACSQuant Analyzer 16 simplify your data acquisition. Learn how expanded fluorescent capabilities, violet side scatter for small particle analysis, and renowned automation can maximize your productivity and discovery in flow cytometry assays. Fluorescent channels are available with 405, 488, and 640 nm excitation lasers to cover a broad range of dyes and fluorescent proteins, enabling you to tell your story with more color. Request the presentation or watch the recording. (August 2018)

Multicolor flow cytometry – Understand the basics to be the expert

The aim of this 45-minute webinar by Guerric Epron, Miltenyi Biotec, is to explain technical basics and how they affect multicolor flow cytometry: Why is a fluorochrome bright or dim? How do you compensate? What is the data-spread effect and can you overcome it? Understand the essential steps for high quality results and interpretation - accurate selection of fluorochromes, consideration of antigen density and setting your flow cytometer properly. Request the presentation or watch the recording. (March 2017)

Recombinant antibodies for improved reproducibility in flow cytometry analysis

Antibodies are key reagents for numerous cell analysis applications. Recently, there were a lot of discussions on irreproducible results due to the inconsistency of antibodies. Recombinantly generated antibodies, such as the REAfinity™ Antibodies, possess highly defined characteristics conditions leading to reproducible results. Request the presentation (pdf) or watch the webinar to understand the common problems associated with antibodies in flow cytometry and the advantages of REAfinity Antibodies. (March 2017)

Exosome analysis using a multiplex bead-based flow cytometry assay

Stefan Wild, senior scientist R&D at Miltenyi Biotec introduces in this webinar a multiplex bead-based flow cytometry assay for exosome analysis. Watch the webinar and see how easy EVs can be distinguished by surface marker profiling and how selected markers can be used to separate EV populations for further downstream analysis. Watch the webinar recording or request the presentation (pdf). (April 2016)

How to optimize sample preparation for flow cytometry

Careful preparation of cell samples is the first crucial step towards reliable flow cytometric analysis. Suspensions of single, viable cells are a prerequisite for obtaining optimal results. In this technical presentation we will discuss how to prepare samples from diverse specimens like cultured cells, blood, or tissue. Request the presentation (pdf) or watch the webinar recording. (January 2016)

Analysis of intracellular signaling using flow cytometry

Please request the presentation or watch the recording to learn how to set up a successful phospho-flow analysis. In this 1h webinar we will present already optimised reagents that will allow you an easy and fast analysis of intracellular signaling. (December 2015)

Multicolor Flow Cytometry

Multicolor flow cytometric analysis of cells provides a wealth of information from even small samples. This webinar explains how to optimally apply compensation to multicolor analysis and which controls have to be done. Request the presentation or watch the recording. (July 2015)

MACSPlex Cytokine – Learn how to simplify your cytokine measurement

MACSPlex Cytokine assays analyze a large number of cells in a very short timeframe and yield highly significant statistical data in terms of cytokine expression and secretion. Lern more about MACSPlex Cytokine assay setup, instrument setup, and the proper use of standards. Furthermore, we will introduce you to the automated analysis features of our MACSPlex Cytokine Express modes. Request the presentation (pdf) or watch the webinar recording. Presenter: Katrin Lange, R&D, Miltenyi Biotec GmbH (June 2015)

Rare cell analysis in flow cytometry

This webinar highlights how to set up rare cell experiments in flow cytometry, including sample labeling and instrument setup. Additional topics include how to overcome common challenges and extend the detection limit of conventional flow analysis using pre-enrichment. Please request the presentation or watch the recording. (October 2014)

Get the colors right: Compensation of spectral overlap in flow cytometry

Multicolor flow cytometric analysis of cells provides a wealth of information from even small samples. However, spectral overlap can cause false positive events. Compensation can be applied to overcome this problem and help generate accurate data. This webinar explains how to optimally apply compensation to multicolor analysis. Using examples of typical fluorochromes, specific compensation issues and their resolution will be addressed. Request the presentation or watch the recording. (February 2014) 

Save time and effort in your flow cytometry experiments

Shorten assay set up and problem solving time with reagent kits and cocktails designed with the busy scientist in mind. Take advantage of flow cytometry reagents, kits and assays that are fast and easy to implement in your every day work process and that enable sensitive rare cell analysis. To learn more about comprehensive flow cytometry solutions, request the presentation (pdf) or watch the webinar recording. (July 2011)

GMP 101 – Introduction to GMP basics

In the first webinar of the “GMP for translational research” series we will give an introduction to the basic principles of good manufacturing practice (GMP), relevant international norms and an introduction to GMP manufacturing of advanced therapies medicinal products (ATMP). Request the presentation (pdf) or watch the recording. (April 2016)

The autoMACS® Pro Separator – the gold standard in walkaway cell separation

With thousands of instruments in use worldwide, the autoMACS Pro Separator provides standardized walkaway cell isolation that is gentle to your cells. In this webinar we explain how to achieve automated multisample cell labeling and separation of viable cells. Using examples from several applications, including whole blood procedures in multiple myeloma and chimerism studies, the versatility of the instrument will be demonstrated. Request the presentation or watch the recording. (March 2014)

Successful cell analysis – optimize the basic steps

Join Dr. Frank Hardung in this webinar on easy preparation of cell samples, magnetic labeling and separation of cells, and quality control of cell separations. Optimizing these basic steps is a prerequisite to successful cell analysis. Request the presentation or watch the recording. (February 2013)