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With our online tutorials, you can start, pause, and stop your courses whenever you want – a convenient way for all levels of users to stay up to date in their field.

Take your on-demand tutorial today, or feel free to contact us directly with any questions or requests: MACS_Academy@miltenyibiotec.de

Immunology research

T cell culture with Miltenyi Biotec

Cell culture is key for efficient and reliable T cell workflows. Miltenyi Biotec offers a large range of cell culture products optimized for T cell research. This 45-minute webinar provides a comprehensive overview of our T cell culture solutions, including specialized T cell media, cytokines, as well as activation and expansion reagents, from research up to MACS® GMP Grade.

Watch the webinar recording or request the presentation (pdf). (November 2019)

Automated manufacturing of CAR NK cells

Learn about the current status of CAR engineered NK cells as an adoptive, cell-based immunotherapy, alongside the strategies developed for CAR NK cell generation.

Watch the webinar recording or request the presentation (pdf). (September 2019)

All you need for immuno-oncology B cell research

Join us to learn about the role of B cell immunotherapies, and therapeutic antibodies in immuno-oncology research.

Watch the webinar recording or request the presentation (pdf). (September 2019)

Single-cell immune profiling of whole blood with a 37-marker mass cytometry assay

In this webinar, we describe our development and optimization of a 37-marker lyophilized antibody panel to profile whole blood samples in a multisite pediatric allergy immune monitoring study. We will discuss several important considerations, such as ensuring optimal data quality, including antibody target and clone selection panel development, and optimization of a streamlined sample processing and data analysis pipeline.

Watch the webinar recording or request the presentation (pdf). (July 2019)

Improve your CAR T cell research

The treatment of cancer patients with CAR T cells is one of the most promising adoptive cellular therapy approaches. Therefore it is vital to be able to provide reliable and trustworthy research materials alongside an easily translatable workflow into the clinic. This webinar explores Miltenyi Biotec’s innovative tools to support CAR T cell research at every step. The workflow covers fast and gentle isolation of donor T cells, efficient T cell activation, gene transfer, and expansion as well as T cell phenotyping and functional analysis of the final CAR T cells.

Watch the webinar recording or request the presentation (pdf). (July 2019)

Working with antigen-specific T cells

In this webinar, Marcello Stein Ph.D., introduces innovative products and solutions for enrichment, stimulation and analysis of rare antigen-specific T cells.

Watch the webinar recording or request the presentation (pdf). (November 2018)

StraightFrom® MicroBeads-Isolation of immune cells using StraightFrom MicroBeads without density gradient

Learn from this webinar how to isolate immune cells straight from blood or blood products without density gradient centrifugation.

Watch the webinar recording or request the presentation (pdf). (June 2018)

Tools for Myeloid Cell Research - The Classical, the Non-Classical and the Macrophage
Join Dr. Emiliano Melandri and his webinar to learn about optimized tools for the study of myeloid cells from both murine models and human samples. A special focus will be on tissue resident and tumor-associated myeloid cell separation strategies. In addition, you will be introduced to the latest products for human monocyte research.

Watch the webinar recording or request the presentation (pdf). (February 2018)

Challenges in NK cell immunotherapy: where do we stand?

This webinar will review the current status of NK cell-based immunotherapy. Martha Elia Luevano Salinas, product manager NK cells, will discuss different strategies used to generate or isolate NK cells for immunotherapy and current approaches boosting NK cell cytotoxicity.

Please request the presentation or watch the webinar recording (October 2016).

Dendritic cells (DC) therapy reloaded

This episode of the translational webinar series reviews the relevance of DC-based therapy and shows innovative tools to develop therapeutic approaches based on primary DCs. Emiliano Melandri, PhD, covers state-of-the-art tools for vaccine development up to complete solutions for DC subset research.

Please request the presentation or watch the webinar recording (October 2016).

Identification and kinetics of circulating follicular human T helper subpopulations

Watch in this webinar how the MACSQuant® Instrument, with its integrated pre-enrichment column, allowed Dr. Elke Bergmann to analyse in detail rare antigen specific CXCR5⁺ T cells after vaccine administration, gaining novel insights into the fight against Ebola.

Please request the presentation or watch the webinar recording. (September 2016)

Dendritic cells: all you need is blood

Dendritic cells (DCs) are a type of leukocytes that play a key role in our immune system. They link the innate and adaptive immunity and are the most potent antigen-presenting cell type. This webinar explains how to investigate peripheral blood derived DC and give an overview of DC subsets in human peripheral blood. Watch the webinar recording or request the presentation. (September 2015)

Stem cell research

Isolation and expansion of MSCs for translational and clinical

Learn in this webinar about MSC expansion, the CliniMACS Prodigy® Adherent Cell Culture System to acquire GMP-compliant and clinical-scale MSCs, and fast and easy characterization of MSCs via flow cytometry.

Watch the webinar recording or request the presentation (pdf) (April 2019)

How to improve sperm cell selection for in vitro fertilization

Learn about the MACS® ART Annexin V System – the most innovative technique to select non-apoptotic sperm cells for in vitro fertilization (IVF).

Watch the webinar recording or request the presentation (pdf) (October 2018)

Generation of highly enriched hiPSC-CMs for high-throughput cardiotoxicity screens

Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) are emerging as an ideal in vitro platform for pre-clinical cardiotoxicity screening of new chemical entities. In this webinar, Todd J. Herron, PhD, Director of the Cardiovascular Regeneration Core Laboratory, University of Michigan, shows how they utilized a non-genetic approach to generate highly enriched hiPSC-CMs using magnetic cell sorting to deplete non-cardiomyocytes from the cell population.

Watch the recording or request the presentation of this webinar. (June 2017)

Foundations of stem cell maintenance

In this webinar, we’ll guide you through the different opportunities to culture cells and test their quality. Learn more about cell splitting techniques and find out how to sort pluripotent stem cells for continuous, high-level quality.

Request the presentation or watch the webinar recording. (April 2016)

Foundations for stem cell reprogramming

Foundations for stem cell reprogramming is the first in a series of webinars on stem cell research. The 45-minute webinar introduces a highly efficient method for reprogramming into iPS cells and will deepen your stem cell knowledge. Request the presentation or watch the webinar recording. (August 2015)

Neuroscience research

Tools for astrocyte characterization, isolation, and cultivation

Learn from this webinar how to reliably characterize neonatal and adult astrocytes with novel astrocyte-specific monoclonal antibodies targeting astrocyte cell surface antigen-1 (ACSA-1) and -2 (ACSA-2). Anti-ACSA-1 (GLAST) and Anti-ACSA-2 antibodies are suitable for astrocyte isolation, as well as IHC/ICC and flow cytometry analysis – the perfect tool for astrocyte characterization.

Request the presentation or watch the webinar recording. (August 2018)

Adult brain dissociation and neural cell isolation

Join Hui Zhang, PhD, and her webinar on the latest options and integrated solutions for neuroscience research, and the use of MACS Technology for adult brain dissociation and neural cell isolation.

Request the presentation or watch the webinar recording. (July 2017)

Cancer

Enhance your T cell flow analysis in immuno-oncology

With immunotherapies being more frequently selected as a cancer treatment, the need for further T cell focused cancer research is increasing. Flow cytometry is considered as the favored method of choice to monitor phenotype, plasticity and functionality of T cells. However, experiment planning and data analysis can be challenging due to time-consuming panel design and choosing the correct gating strategy. Furthermore, inconsistency in results may also be influenced by inter-operator variability and reagent quality.

Watch the webinar recording or request the presentation (pdf). (December 2019)

Tumor cell preparation for single cell sequencing

Learn from this webinar how the preparation of single cells from tumor samples influences the performance and reproducibility of your single cell analysis. In collaboration with 10X Genomics, we dissociated tumor tissue with the gentleMACS™ Octo Dissociator with Heaters and the Tumor Dissociation Kit, mouse before analyzing the samples using the Chromium™ System. We cover several aspects of tumor cell analysis regarding the performance of the overall workflow, reproducibility, and the characterization of different cell populations.

Watch the webinar recording or request the presentation (pdf). (July 2018)

Isolation of carcinoma cells from FFPE samples for high-quality sequencing results
In this webinar, Bastian Nicolai introduces an innovative method for dissociation of FFPE carcinoma samples into single cell suspensions for high-quality sequencing results. See how the new FFPE Tissue Dissociation Kit from Miltenyi Biotec, also suited for any other tissue from any species, preserves cytoskeletal markers and improves molecular analysis. Dissociated cells can be subsequently separated by flow sorting or analyzed by flow cytometry in vitro for phenotype distributions, and other genetic or proteomic studies.

Watch the webinar recording or request the presentation (pdf). (June 2018)

Isolation of tumor cells and TILs for optimized downstream analysis

For the development of novel immune-based therapeutic strategies, it is essential to study the interaction and phenotypes of immune and tumor cells within the tumor microenvironment in detail. In this webinar, Dr. Olaf Hardt introduces a sophisticated workflow that allows for the isolation of tumor cell subpopulations and tumor-infiltrating leukocytes (TILs). You will learn about optimized techniques for the dissociation of tumor tissue as well as the analysis of tumor cells and tumor-infiltrating immune cell subpopulations by flow cytometry.

Watch the webinar recording or request the presentation (pdf). (May 2018)

Tumor microenvironment - Tools for TIL isolation and flow cytometry analysis
A deep understanding of the interactions and phenotypes of immune and tumor cells within the tumor microenvironment is key to developing future immune-based cancer therapy strategies. In this webinar, Dr. César Evaristo describes best practices for isolation of tumor infiltrating leukocytes (TILs) and present an optimized workflow for faster and improved flow analysis. You will be introduced to innovative tools and solutions to increase quality of data, reduce experimental time, and ease scale-up during TIL analysis using flow cytometry.

Watch the webinar recording or request the presentation (pdf). (May 2018)

Advancing tumor research: a revolutionary workflow for isolating pure cell populations from primary tumor tissue

Tumor tissue is infiltrated by non-tumor cells (TILs) in such a way, that the comparison of even two identical tumor types in the same tissue will show very high differences in levels of infiltration. To make these samples comparable and useful for any downstream application, it is crucial to have pure cell populations of your cells of interest. In this webinar we will explain the complete workflow for dissociating human, mouse and xenograft tissue into single cell suspensions and show how to specifically separate tumor cells from non-tumor cells.

Watch the webinar recording or request the presentation (pdf). (June 2017)

Flow cytometry & sorting

Genetic toxicology testing and DNA damage response

Watch the webinar to learn about a review of semi-automated products and procedures for higher throughput genetic toxicology testing and DNA damage response.

Watch the webinar recording or request the presentation (pdf). (November 2019)

Immunogenicity testing – drug development

Watch the webinar to learn about immunogenicity testing at the early phase of drug development. Find out how it can play a crucial role in avoiding possible clinical consequences of unwanted immune responses.

Watch the webinar recording or request the presentation (pdf). (July 2019)

Reproducible flow cytometry data with lot-to-lot consistent recombinant antibodies

In the final webinar of this series, the following topics will be discussed: • Antibody-related reproducibility crisis and implications for antibody-based quality controls in cell manufacturing • Advances in recombinant antibody technology and opportunities for flow cytometry assays • Introduction of REAfinity™ Recombinant Antibodies and exemplary data in cell manufacturing assays

Watch the webinar recording or request the presentation (pdf). (July 2019)

Getting flow cytometer calibration right – Harmonization of multiple instruments with Smart Gain technology

In this video, we review some common challenges in harmonization of flow cytometer settings across different devices and sites, cover various instrument calibration tips, and introduce Smart Gain technology for seamless harmonization efforts.

Watch the webinar recording or request the presentation (pdf). (June 2019)

Automated sample acquisition and analysis of cell products with Express Mode software

Flow cytometry-based analysis of cell products can be a challenging task, when you don´t have systems in place that deliver consistent data over time. In the first of three webinars the following topics will be discussed: 1. Common challenges in the monitoring and release testing of cell products, such as CAR T cells 2. Solutions to improve standardization of assays and consistency of results 3. Introduction of Express Modes for automated sample acquisition and analysis during cell manufacturing. Watch the webinar recording or request the presentation (pdf). (April 2019)

REAlease® Releasable Antibodies for label free cells after cell sorting

In this webinar learn about our brand-new REAlease Fluorochrome Technology. In just one step, it allows for removal of any labels from cells after cell sorting. Find out how this next step in flexibility could open up new possibilities for your downstream applications. Watch the webinar recording or request the presentation (pdf). (October 2018)

The MACSQuant® Tyto® Cell Sorter – Gentle and sterile cell sorting in a closed cartridge system
In this webinar, you will learn about microchip-based cell sorting on the MACSQuant® Tyto® Cell Sorter. Find out how cell sorting within a closed cartridge system allows for a more gentle and sterile approach than conventional droplet sorting. Watch the webinar recording or request the presentation (pdf). (October 2018)

The MACSQuant® Analyzer 16 – Colorful new possibilities in automated flow cytometry

As research questions become more complex, let the MACSQuant Analyzer 16 simplify your data acquisition. Learn how expanded fluorescent capabilities, violet side scatter for small particle analysis, and renowned automation can maximize your productivity and discovery in flow cytometry assays. Fluorescent channels are available with 405, 488, and 640 nm excitation lasers to cover a broad range of dyes and fluorescent proteins, enabling you to tell your story with more color. Request the presentation or watch the recording. (August 2018)

Multicolor flow cytometry – Understand the basics to be the expert

The aim of this 45-minute webinar by Guerric Epron, Miltenyi Biotec, is to explain technical basics and how they affect multicolor flow cytometry: Why is a fluorochrome bright or dim? How do you compensate? What is the data-spread effect and can you overcome it? Understand the essential steps for high quality results and interpretation - accurate selection of fluorochromes, consideration of antigen density and setting your flow cytometer properly. Request the presentation or watch the recording. (March 2017)

Recombinant antibodies for improved reproducibility in flow cytometry analysis

Antibodies are key reagents for numerous cell analysis applications. Recently, there were a lot of discussions on irreproducible results due to the inconsistency of antibodies. Recombinantly generated antibodies, such as the REAfinity™ Antibodies, possess highly defined characteristics conditions leading to reproducible results. Request the presentation (pdf) or watch the webinar to understand the common problems associated with antibodies in flow cytometry and the advantages of REAfinity Antibodies. (March 2017)

Exosome analysis using a multiplex bead-based flow cytometry assay

Stefan Wild, senior scientist R&D at Miltenyi Biotec introduces in this webinar a multiplex bead-based flow cytometry assay for exosome analysis. Watch the webinar and see how easy EVs can be distinguished by surface marker profiling and how selected markers can be used to separate EV populations for further downstream analysis. Watch the webinar recording or request the presentation (pdf). (April 2016)

How to optimize sample preparation for flow cytometry

Careful preparation of cell samples is the first crucial step towards reliable flow cytometric analysis. Suspensions of single, viable cells are a prerequisite for obtaining optimal results. In this technical presentation we will discuss how to prepare samples from diverse specimens like cultured cells, blood, or tissue. Request the presentation (pdf) or watch the webinar recording. (January 2016)

Analysis of intracellular signaling using flow cytometry

Please request the presentation or watch the recording to learn how to set up a successful phospho-flow analysis. In this 1h webinar we will present already optimised reagents that will allow you an easy and fast analysis of intracellular signaling. (December 2015)

Multicolor Flow Cytometry

Multicolor flow cytometric analysis of cells provides a wealth of information from even small samples. This webinar explains how to optimally apply compensation to multicolor analysis and which controls have to be done. Request the presentation or watch the recording. (July 2015)

MACSPlex Cytokine – Learn how to simplify your cytokine measurement

MACSPlex Cytokine assays analyze a large number of cells in a very short timeframe and yield highly significant statistical data in terms of cytokine expression and secretion. Lern more about MACSPlex Cytokine assay setup, instrument setup, and the proper use of standards. Furthermore, we will introduce you to the automated analysis features of our MACSPlex Cytokine Express modes. Request the presentation (pdf) or watch the webinar recording. Presenter: Katrin Lange, R&D, Miltenyi Biotec B.V. & Co. KG (June 2015)

Rare cell analysis in flow cytometry

This webinar highlights how to set up rare cell experiments in flow cytometry, including sample labeling and instrument setup. Additional topics include how to overcome common challenges and extend the detection limit of conventional flow analysis using pre-enrichment. Please request the presentation or watch the recording. (October 2014)

Get the colors right: Compensation of spectral overlap in flow cytometry

Multicolor flow cytometric analysis of cells provides a wealth of information from even small samples. However, spectral overlap can cause false positive events. Compensation can be applied to overcome this problem and help generate accurate data. This webinar explains how to optimally apply compensation to multicolor analysis. Using examples of typical fluorochromes, specific compensation issues and their resolution will be addressed. Request the presentation or watch the recording. (February 2014)

Technology & automation

Whole organ quantitative biology using light sheet microscopy

In this webinar, Prof. Dr. Matthias Gunzer presents new approaches in quantification of glomeruli in murine kidneys under normal and pathological conditions. He discusses impacts of ischemic insult on the vascular structure in affected heart muscles and shows how tissue clearing and light sheet microscopy has been fundamental for the discovery of a new type of blood vessels in murine and human long bones.

Request the presentation (pdf) or watch the recording. (July 2019)

Automation meets cell separation

No matter the specific application, primary cells are essential for the discovery of new therapeutics, which means reliable sample processing, including cell separation and tumor dissociation, is paramount. This webinar will take you through our flexible, automated solutions for primary cell isolation from various starting materials. Join us and learn about the best combination of instruments and reagents for your research.

Request the presentation (pdf) or watch the recording. (July 2019)

GMP-compliant multiparametric cell sorting

In this webinar, you will learn about the unique microchip-based cell sorting technology of the MACSQuant® Tyto® Cell Sorter. Find out how this GMP-compliant system can enable an easy transfer of sorting protocols from research into clinical settings.

Request the presentation (pdf) or watch the recording. (May 2019)

The basics of magnetic cell separation with MACS® Technology
In this webinar delivered by Kristina Kremer you will learn the basics of MACS® Technology for magnetic cell separation and discover why it is the best choice for isolation of viable and functional cells. Get to know all solutions of the MACS Cell Separation portfolio providing a broad range of reagents, instruments, and strategies for the fast and gentle isolation of cells from virtually any cell type.

Request the presentation or watch the webinar recording.(April 2019)

The CliniMACS Prodigy Customized Application Service

Join Dr. Peter Jähn and his webinar on the CliniMACS Prodigy Platform, including features, benefits, and workflow examples. The second part of the webinar focuses on the Customized Application (CAP) Service, which provides tailor-made, automated procedures for your specific cell manufacturing application.

Request the presentation or watch the webinar recording. (November 2017)

GMP 101 – Introduction to GMP basics

In the first webinar of the “GMP for translational research” series we will give an introduction to the basic principles of good manufacturing practice (GMP), relevant international norms and an introduction to GMP manufacturing of advanced therapies medicinal products (ATMP).

Request the presentation (pdf) or watch the recording. (April 2016)

Successful cell analysis – optimize the basic steps

Join Dr. Frank Hardung in this webinar on easy preparation of cell samples, magnetic labeling and separation of cells, and quality control of cell separations. Optimizing these basic steps is a prerequisite to successful cell analysis.

Request the presentation or watch the recording. (February 2013)