Name: Treg Detection Kit (CD4/CD25/CD127), human
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Product data and images for Treg Detection Kit (CD4/CD25/CD127), human

Figures

Figure 1

Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4

⁺

CD25

^high

CD127

^dim/^–

cells.

A:	B:
An appropriate threshold was defined based on CD45-VioBlue versus side scatter signals, for the exclusion of debris and erythrocytes from the data.	Lymphocytes were gated for their expected morphology using a forward scatter versus side scatter "lymphocyte gate" (P1)
View details Figure 1 Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 ⁺ CD25 ^high CD127 ^dim/^– cells.	View details Figure 1 Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 ⁺ CD25 ^high CD127 ^dim/^– cells.
C:	D:
Dead cells were excluded by a CD127-PE versus propidium iodide dot plot (P2).	CD4 ⁺ lymphocytes were gated in P3.
View details Figure 1 Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 ⁺ CD25 ^high CD127 ^dim/^– cells.	View details Figure 1 Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 ⁺ CD25 ^high CD127 ^dim/^– cells.
CD25 ^high CD127 ^dim/– Treg cells were gated by a CD127-PE versus CD25-APC dot plot.
View details Figure 1 Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 ⁺ CD25 ^high CD127 ^dim/^– cells.

Specifications for Treg Detection Kit (CD4/CD25/CD127), human

Overview

The Treg Detection Kit (CD4/CD25/CD127) has been developed as a simple one-reagent solution for the identification of human regulatory T (Treg) cells by flow cytometry. Applications include

Staining and enumeration of CD4⁺CD25⁺CD127^dim/– regulatory T cells in PBMCs, apheresis products, umbilical cord blood, or whole blood
Analysis of Treg cells separated using MACS® Technology

Detailed product information

Background information

The kit combines monoclonal antibodies against the standard Treg cell markers CD4, CD25, and CD127 as well as the leukocyte marker CD45 at optimized titers in one vial. The combination of CD4, CD25, and CD127 staining is the current standard method for the identification of live Treg cells. Several publications have reported a strong correlation between the CD25

⁺

CD127

^dim/–

phenotype, expression of FoxP3, and suppressive function in CD4

⁺

cells.

^1,2

Resources for Treg Detection Kit (CD4/CD25/CD127), human

Documents and Protocols

Brochures/posters

Regulatory T cell research

References for Treg Detection Kit (CD4/CD25/CD127), human

Publications

Seddiki, N. et al. (2006) Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells. J. Exp. Med. 203: 1693-1700
Liu, W. et al. (2006)
CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4
⁺
T reg cells.
J. Exp. Med. 203: 1701-1711

Data and images

Data and images for Treg Detection Kit (CD4/CD25/CD127), human

Figures

Figure 1

⁺

CD25

^high

CD127

^dim/^–

cells.

A:	B:
An appropriate threshold was defined based on CD45-VioBlue versus side scatter signals, for the exclusion of debris and erythrocytes from the data.	Lymphocytes were gated for their expected morphology using a forward scatter versus side scatter "lymphocyte gate" (P1)
View details Figure 1 Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 ⁺ CD25 ^high CD127 ^dim/^– cells.	View details Figure 1 Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 ⁺ CD25 ^high CD127 ^dim/^– cells.
C:	D:
Dead cells were excluded by a CD127-PE versus propidium iodide dot plot (P2).	CD4 ⁺ lymphocytes were gated in P3.
View details Figure 1 Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 ⁺ CD25 ^high CD127 ^dim/^– cells.	View details Figure 1 Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 ⁺ CD25 ^high CD127 ^dim/^– cells.
CD25 ^high CD127 ^dim/– Treg cells were gated by a CD127-PE versus CD25-APC dot plot.
View details Figure 1 Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 ⁺ CD25 ^high CD127 ^dim/^– cells.

Specifications

Specifications for Treg Detection Kit (CD4/CD25/CD127), human

Overview

The Treg Detection Kit (CD4/CD25/CD127) has been developed as a simple one-reagent solution for the identification of human regulatory T (Treg) cells by flow cytometry. Applications include

Staining and enumeration of CD4⁺CD25⁺CD127^dim/– regulatory T cells in PBMCs, apheresis products, umbilical cord blood, or whole blood
Analysis of Treg cells separated using MACS® Technology

Detailed product information

Background information

⁺

CD127

^dim/–

phenotype, expression of FoxP3, and suppressive function in CD4

⁺

cells.

^1,2

Resources

Resources for Treg Detection Kit (CD4/CD25/CD127), human

Documents and Protocols

Brochures/posters

Regulatory T cell research

References

References for Treg Detection Kit (CD4/CD25/CD127), human

Publications

Seddiki, N. et al. (2006) Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells. J. Exp. Med. 203: 1693-1700
Liu, W. et al. (2006)
CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4
⁺
T reg cells.
J. Exp. Med. 203: 1701-1711

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Treg Detection Kit (CD4/CD25/CD127), human

Treg Detection Kit (CD4/CD25/CD127), human

Product overview

Product data and images for Treg Detection Kit (CD4/CD25/CD127), human

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for Treg Detection Kit (CD4/CD25/CD127), human

Overview

Detailed product information

Background information

Resources for Treg Detection Kit (CD4/CD25/CD127), human

Documents and Protocols

Regulatory T cell research

References for Treg Detection Kit (CD4/CD25/CD127), human

Publications

Data and images

Data and images for Treg Detection Kit (CD4/CD25/CD127), human

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications

Specifications for Treg Detection Kit (CD4/CD25/CD127), human

Overview

Detailed product information

Background information

Resources

Resources for Treg Detection Kit (CD4/CD25/CD127), human

Documents and Protocols

Regulatory T cell research

References

References for Treg Detection Kit (CD4/CD25/CD127), human

Publications

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Treg Detection Kit (CD4/CD25/CD127), human