Clone:
REA559
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
TCR Vβ-11, V beta 11 TCR

Extended validation for TCR Vβ11 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA559
c21+
Cells were incubated with an excess of purified unconjugated TCR Vβ11 (REA559) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for TCR Vβ11. Human peripheral blood mononuclear cells (PBMCs) were stained with TCR Vβ11 antibodies and with a suitable counterstaining. As a control, TCR Vβ11 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for TCR Vβ11. Human peripheral blood mononuclear cells (PBMCs) were stained with TCR Vβ11 antibodies and with a suitable counterstaining. As a control, TCR Vβ11 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for TCR Vβ11. Human peripheral blood mononuclear cells (PBMCs) were stained with TCR Vβ11 antibodies and with a suitable counterstaining. As a control, TCR Vβ11 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for TCR Vβ11. Human peripheral blood mononuclear cells (PBMCs) were stained with TCR Vβ11 antibodies and with a suitable counterstaining. As a control, TCR Vβ11 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using TCR Vβ11 (REA559). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using TCR Vβ11 (REA559). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using TCR Vβ11 (REA559). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for TCR Vβ11 Antibody, anti-human, REAfinity™

Overview

Clone REA559 recognizes the human Vβ11 T cell receptor (TCR Vβ11). The TCR is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a disulfide-linked membrane-anchored heterodimeric glycoprotein normally consisting of the highly variable α and β chains expressed as part of a complex with the invariant CD3 chain molecules. TCR Vβ11 is expressed on a subset of α/β T cells.
Additional information: Clone REA559 displays negligible binding to Fc receptors.

Alternative names

TCR Vβ-11, V beta 11 TCR

Detailed product information

Technical specifications

CloneREA559
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenTCR Vβ11
Alternative names of antigenTCR Vβ-11, V beta 11 TCR
Molecular mass of antigen [kDa]13
Distribution of antigenT cells
Entrez Gene ID6957
RRIDAB_2811534, AB_2857782, AB_2857773, AB_2653742, AB_2653743, AB_2811540

Resources for TCR Vβ11 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for TCR Vβ11 Antibody, anti-human, REAfinity™

Publications

  1. Kurt, R. A. et al. (2000)
    TCR v(beta) usage and clonality of T cells isolated from progressing and rejected tumor sites before and after
    in vitro
    culture.
    Int. Immunol. 12(5): 639-646
  2. Robinson, M. A. et al. (1989) Allelic sequence variations in the hypervariable region of a T-cell receptor beta chain: correlation with restriction fragment length polymorphism in human families and populations. Proc. Natl. Acad. Sci. U.S.A. 86: 9422-9426

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