Type of antibody:
Primary antibodies, Recombinant antibodies
recombinant human IgG1
Alternative names:

Extended validation for Myosin Heavy Chain Antibody, anti-human/mouse/rat, REAfinity™


Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA399
3-48 GSC7-
Cells were incubated with an excess of purified unconjugated Myosin Heavy Chain (REA399) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for Myosin Heavy Chain Antibody, anti-human/mouse/rat, REAfinity™


Clone REA399 recognizes the human, mouse, and rat cardiac isoform of the myosin heavy chain (MHC) antigen. Myosin forms cross-bridges with actin in cardiac, smooth, and skeletal muscle, and cyclic cross-bridge activity is the basis for muscular contraction. It is a hexamer composed of two heavy chains, each of which contains an actin-binding site and an ATP hydrolysis site, and four light chains. Isoforms of each myosin subunit are expressed in cardiac, skeletal, and smooth muscle, and many are associated with distinct contractile properties in the muscles or single muscle fibers in which they are expressed. In cardiac muscle, two MHC isoforms, termed α-MHC and β-MHC, are present. α-MHC is associated with higher actomyosin ATPase activity compared with β-MHC. The relative amounts of the two isoforms may vary, with the ratio being a function of the hormonal status, the developmental stage, the animal, or the particular region of the heart.
Additional information: Clone REA399 displays negligible binding to Fc receptors.

Alternative names


Detailed product information

Technical specifications

Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse, rat, other
Cross-reactivityrabbit, cow, dog, pig
AntigenMyosin Heavy Chain
Alternative names of antigenMYH6, MYH7, MHC, MYHC
Molecular mass of antigen [kDa]223(average of subunits)
Distribution of antigencardiac muscle, skeletal muscle, smooth muscle
Entrez Gene ID4624, 4625
RRIDAB_2889714, AB_2652979, AB_2652980, AB_2652981, AB_2889715

Resources for Myosin Heavy Chain Antibody, anti-human/mouse/rat, REAfinity™


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References for Myosin Heavy Chain Antibody, anti-human/mouse/rat, REAfinity™


  1. Cosper, P. F. et al. (2012) Interferon-γ causes cardiac myocyte atrophy via selective degradation of myosin heavy chain in a model of chronic myocarditis. Am. J. Pathol. 181(6): 2038-2046
  2. Haddad, F. et al. (2010) Cardiac myosin heavy chain gene regulation by thyroid hormone involves altered histone modifications. Am. J. Physiol. Heart Circ. Physiol. 299(6): H1968-H1980
  3. Molkentin, J. D. et al. (1996) Alpha-myosin heavy chain gene regulation: delineation and characterization of the cardiac muscle-specific enhancer and muscle-specific promoter. J. Mol. Cell. Cardiol. 28(6): 1211-1225

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