Alternative names:
TNFSF2

Data and images for Mouse TNF-α

Figures

Figure 1

View details
Mouse TNF-α activity assay.
The biological activity of Mouse TNF-α, premium grade, was determined by inhibition assay using L-929 cells. Activity of Mouse TNF-α, premium grade, (red line) was compared to another commercially available product (black line).

Figure 1

Mouse TNF-α activity assay.
The biological activity of Mouse TNF-α, premium grade, was determined by inhibition assay using L-929 cells. Activity of Mouse TNF-α, premium grade, (red line) was compared to another commercially available product (black line).

Figure 2

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SDS-PAGE of Mouse TNF-α, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Mouse TNF-α, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 3

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Mass spectrometry analysis (ESI-MS) of Mouse TNF-α, premium grade. The Peak corresponds to the calculated molecular mass of 17256 Da.

Figure 3

Mass spectrometry analysis (ESI-MS) of Mouse TNF-α, premium grade. The Peak corresponds to the calculated molecular mass of 17256 Da.

Specifications for Mouse TNF-α

Overview

TNF-α stands for tumor necrosis factor α, also termed TNFSF2 (TNF ligand superfamily member 2) or Cachectin. Mouse TNF-α is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse TNF-α can be used for a variety of applications, including:
  • Activation of antigen presenting cells.
  • Cytotoxicity and cell proliferation assays.
  • Assessment of apoptosis and viral protection.
  • Investigation of TNF-α–induced signaling pathways.

Alternative names

TNFSF2

Detailed product information

Background information

Tumor necrosis factor α (TNF-α) is a proinflammatory cytokine mainly produced by activated monocytes and macrophages in response to infection, injury, and tumor burden. TNF-α production has also been reported for a variety of other cell types involved in inflammatory responses, including T cells, NK cells, and neutrophils as well as a number of non-immune cells, such as keratinocytes and astrocytes. TNF-α has a broad spectrum of biological activities. In addition to its central role in inflammation, TNF-α is noted for its cytotoxic and tumoricidal abilities either by necrosis or induction of apoptosis. Further functions include antiviral activity, growth modulation, and induction of cellular differentiation. Despite its various beneficial actions, TNF-α also plays a detrimental role in, for example, septic shock syndrome, tissue injury, inflammation, cachexia, and diabetes.

Biological activity

  • Inhibition of L-929 cells in the presence of actinomycin D
  • premium grade: ≥ 8×
    10
    7
    U/mg
  • research grade: ≥ 4×
    10
    7
    U/mg

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Mouse TNF-α

Documents and Protocols

Background information

Premium-grade cytokine benefits

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Mouse TNF-α

Publications

  1. Baarsch, M. J. et al. (1991) Detection of tumor necrosis factor alpha from porcine alveolar macrophages using an L929 fibroblast bioassay. J. Immunol. Methods 140: 15-22

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