Alternative names:
c-kit ligand, steel factor, MGF

Data and images for Mouse SCF

Figures

Figure 1

View details
Mouse SCF activity assay.
The biological activity of Mouse SCF, premium grade, was determined by proliferation assay using TF-1 cells. Activity of Mouse SCF, premium grade, (red line) was compared to another commercially available product (black line).

Figure 1

Mouse SCF activity assay.
The biological activity of Mouse SCF, premium grade, was determined by proliferation assay using TF-1 cells. Activity of Mouse SCF, premium grade, (red line) was compared to another commercially available product (black line).

Figure 2

View details
SDS-PAGE of Human SCF, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Human SCF, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 3

View details
Mass spectrometry analysis (ESI-MS) of Mouse SCF, premium grade. The peak corresponds to the calculated molecular mass of 18425 Da.

Figure 3

Mass spectrometry analysis (ESI-MS) of Mouse SCF, premium grade. The peak corresponds to the calculated molecular mass of 18425 Da.

Specifications for Mouse SCF

Overview

SCF stands for stem cell factor, also termed c-Kit ligand. Mouse SCF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse SCF may be used for a variety of applications, including:
  • In vitro culture and expansion of hematopoietic progenitor cells.
  • Colony formation assays.
  • Differentiation of ES-derived cells towards the hematopoietic lineage.
  • Mast cell differentiation and maintenance.
  • Investigation of receptor signaling.

Alternative names

c-kit ligand, steel factor, MGF

Detailed product information

Background information

Stem cell factor (SCF), also known as c-kit ligand, is a hematopoietic growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and progenitor cells. Besides its pivotal role in mast cell development, SCF acts as a potent mast cell chemoattractant and upregulates mast cell adhesion and migration. SCF signals through the c-kit receptor (CD117) and exists in two forms, cell surface bound SCF and soluble SCF. The secreted soluble form of SCF is produced by the proteolytic processing of the cell surface anchored precursor molecule. Recombinant Mouse SCF corresponds to the secreted soluble form.

Biological activity

  • Proliferation of TF-1 cells
  • research grade: ≥ 2×
    10
    4
    U/mg
  • premium grade: ≥ 4×
    10
    4
    U/mg
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Mouse SCF

Documents and Protocols

Background information

Premium-grade cytokine benefits

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Mouse SCF

Publications

  1. Kitumura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
  2. Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
  3. Dussiau, C. et al. (2015) Targeting IRAK1 in T-cell acute lymphoblastic leukemia. Oncotarget. 6: 18956-18965
  4. Brault, J. et al. (2014)
    Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
    ex vivo
    models of X
    0
    -linked, AR22
    0
    - and AR47
    0
    - chronic granulomatous diseases.
    Biores Open Access 3(6): 311-326
  5. Dighe N. et al. (2014) Long-term reproducible expression in human fetal liver hematopoietic stem cells with a UCOE-based lentiviral vector. PLoS One 9(8): e104805
  6. Velardi, E. et al. (2014) Sex steroid blockade enhances thymopoiesis by modulating Notch signaling. J. Exp. Med. 211(12): 2341-2349
  7. Rodriguez, S. et al. (2011) The SKP2 E3 ligase regulates basal homeostasis and stress-induced regeneration of HSCs. Blood 117(24): 6509-6519
  8. Steinleitner, K. et al. (2012) EVI1 and MDS1/EVI1 expression during primary human hematopoietic progenitor cell differentiation into various myeloid lineages. Anticancer Res. 32(11): 4883-4889
  9. Narla, A. et al. (2011) Dexamethasone and lenalidomide have distinct functional effects on erythropoiesis. Blood 118(8): 2296-2304

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