The IFN-γ Secretion Assay was developed for the sensitive detection of mouse IFN-γ-secreting cells.

Data and images for Mouse IFN-γ Secretion Assay – Detection Kits

Figures

Figure 1

In this example, BALB/c mice were immunized i.p. with KLH (keyhole limpet hemocyanin) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for coexpression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (APC) and Mouse IL-2 Secretion Assay - Detection Kit (PE). The plots show coexpression of IFN-γ- and IL-2-secreting cells gated on viable CD4
+
T cells on the stimulated sample as well as on the unstimulated control.
Stimulated sample
Unstimulated control
View details

Figure 1

In this example, BALB/c mice were immunized i.p. with KLH (keyhole limpet hemocyanin) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for coexpression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (APC) and Mouse IL-2 Secretion Assay - Detection Kit (PE). The plots show coexpression of IFN-γ- and IL-2-secreting cells gated on viable CD4
+
T cells on the stimulated sample as well as on the unstimulated control.
View details

Figure 1

In this example, BALB/c mice were immunized i.p. with KLH (keyhole limpet hemocyanin) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for coexpression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (APC) and Mouse IL-2 Secretion Assay - Detection Kit (PE). The plots show coexpression of IFN-γ- and IL-2-secreting cells gated on viable CD4
+
T cells on the stimulated sample as well as on the unstimulated control.

Specifications for Mouse IFN-γ Secretion Assay – Detection Kits

Overview

The IFN-γ Secretion Assay was developed for the sensitive detection of mouse IFN-γ-secreting cells.

Detailed product information

Background information

Interferon-gamma (IFN-γ) is predominantly secreted by activated CD8
+
and CD4
+
memory and effector T cells as well as by NK cells. It is mainly involved in the regulation of inflammatory immune responses. These T
H
1 types of immune mechanisms are effective against intracellular pathogens and tumors and can be involved in immunological disorders, such as autoimmune reactions.

Downstream applications

The Mouse IFN-γ Secretion Assay was used to analyze the sequential production of cytokines by individual staphylococcal enterotoxin B-activated T helper lymphocytes
1
and for studies on T
H
1 cell differentiation
2,3
. Furthermore, it was used for isolation of tumor-reactive T cells from immunized mice for adoptive T cell transfer experiments
4
and from mice immunized with viral antigens.
5
Mouse NKT cells were analyzed
6,7
and isolated
7
using the IFN-γ Secretion Assay after stimulation with glycolipid antigens. IFN-γ expression by T cells was also analyzed for intrahepatic mononuclear cells
8
and cells from bronchioalveolar lavages
9
.

Resources for Mouse IFN-γ Secretion Assay – Detection Kits

References for Mouse IFN-γ Secretion Assay – Detection Kits

Publications

  1. Dana, R. et al. (2016) IL-17 Augments B Cell Activation in Ocular Surface Autoimmunity. J. Immunol. 197(9): 3464-3470
  2. Krilis, S. A. et al. (2016) CD117+ Dendritic and Mast Cells Are Dependent on RasGRP4 to Function as Accessory Cells for Optimal Natural Killer Cell-Mediated Responses to Lipopolysaccharide. PLoS One 11(3)
  3. Assenmacher et al. (1998) Sequential production of IL-2, IFN-gamma and IL-10 by individual staphylococcal enterotoxin B-activated T helper lymphocytes. Eur. J. Immunol. 28: 1534-1543
  4. Wu, C. Y. et al. (2002) Distinct lineages of T(H)1 cells have differential capacities for memory cell generation in vivo. Nat. Immunol. 3: 852-858
  5. Zhang, Y. et al. (2001) Interferon gamma stabilizes the T helper cell type 1 phenotype. J. Exp. Med. 194: 165-172
  6. Becker, C. et al. (2001) Adoptive tumor therapy with T lymphocytes enriched through an IFN-gamma capture assay. Nat Med 7: 1159-1162
  7. Riberdy, J. M. et al. (2001) Cutting edge: culture with high doses of viral peptide induces previously unprimed CD8(+) T cells to produce cytokine. J. Immunol. 167: 2437-2440
  8. Hayakawa et al. (2001) Critical contribution of IFN-gamma and NK cells, but not perforin-mediated cytotoxicity, to anti-metastatic effect of alpha-galactosylceramide. Eur. J. Immunol. 31: 1720-1727
  9. Yang, J. Q. et al. (2003) Immunoregulatory role of CD1d in the hydrocarbon oil-induced model of lupus nephritis. J. Immunol. 171: 2142-2153
  10. Berenzon, D. et al. (2003)
    Protracted protection to
    Plasmodium berghei
    malaria is linked to functionally and phenotypically heterogeneous liver memory CD8
    +
    T cells.
    J. Immunol. 171: 2024-2034
  11. Mc Allister, F. et al. (2004)
    T cytotoxic-1 CD8
    +
    T cells are effector cells against pneumocystis in mice.
    J. Immunol. 172: 1132-1138

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