The MACSxpress
®
Whole Blood Naive B Cell Isolation Kit was developed for the isolation of untouched naive B cells from anticoagulated whole blood without density gradient centrifugation.

Data and images for
MACSxpress
®
Whole Blood Naive B Cell Isolation Kit
, human

Figures

Figure 1

Untouched naive B cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress
®
Whole Blood Naive B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45-VioBlue
®
, CD19-PE-Vio
®
770, CD27-APC, and Anti-IgD-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris, non-leucocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
Unseparated fraction
View details

Figure 1

Untouched naive B cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress
®
Whole Blood Naive B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45-VioBlue
®
, CD19-PE-Vio
®
770, CD27-APC, and Anti-IgD-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris, non-leucocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
View details

Figure 1

Untouched naive B cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress
®
Whole Blood Naive B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45-VioBlue
®
, CD19-PE-Vio
®
770, CD27-APC, and Anti-IgD-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris, non-leucocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
View details

Figure 1

Untouched naive B cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress
®
Whole Blood Naive B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45-VioBlue
®
, CD19-PE-Vio
®
770, CD27-APC, and Anti-IgD-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris, non-leucocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
After separation
View details

Figure 1

Untouched naive B cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress
®
Whole Blood Naive B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45-VioBlue
®
, CD19-PE-Vio
®
770, CD27-APC, and Anti-IgD-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris, non-leucocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
View details

Figure 1

Untouched naive B cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress
®
Whole Blood Naive B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45-VioBlue
®
, CD19-PE-Vio
®
770, CD27-APC, and Anti-IgD-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris, non-leucocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
View details

Figure 1

Untouched naive B cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress
®
Whole Blood Naive B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45-VioBlue
®
, CD19-PE-Vio
®
770, CD27-APC, and Anti-IgD-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris, non-leucocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Specifications for
MACSxpress
®
Whole Blood Naive B Cell Isolation Kit
, human

Overview

The MACSxpress
®
Whole Blood Naive B Cell Isolation Kit was developed for the isolation of untouched naive B cells from anticoagulated whole blood without density gradient centrifugation.

Detailed product information

Detailed separation procedure

With the new MACSxpress
®
Technology, untouched human leukocyte subsets can be isolated from up to 30 mL of anticoagulated whole blood. Non-target cells are removed by immunomagnetic depletion using MACSxpress Beads. Simultaneously, erythrocytes are sedimented, yielding untouched target cells of high purity.

Downstream applications

Naive B cells isolated with the MACSxpress
®
Whole Blood Naive B Cell Isolation Kit can be used, e.g., for phenotyping and functional assays.

Resources for
MACSxpress
®
Whole Blood Naive B Cell Isolation Kit
, human

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MACSxpress
®
Whole Blood Naive B Cell Isolation Kit
, human

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