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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Our local employees are always happy to answer your questions. Highly trained and experienced teams in your country can provide quick, helpful, and comprehensive support.
Miltenyi Biotec B.V.
Sandifortdreef 17
2333 ZZ Leiden
The Netherlands
Phone: 0800 94016
Fax: 0800 99626
E-Mail: macs@miltenyibiotec.nl
Web: www.miltenyibiotec.com
For immediate technical support, use our live chat. Connect with us
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
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Isolation of CD34 + cells from a whole blood sample from a healthy donor using the MACSprep™ Chimerism CD34 MicroBead Kit, two LS Columns, a MACSmix™ Tube Rotator and a MidiMACS™ Separator. Cells were fluorescently stained with CD34-FITC and CD45-VioBlue ® and analyzed by flow cytometry using the MACSQuant ® Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. The purity of CD34 + cells after separation is defined by percentage of CD34 + cells (viable CD45d im/+CD34 + cells) amongst leukocytes (viable CD45 dim/+ cells). The CD34 + cells content of the isolated fraction with one column is typically around 69.1 ± 5.8% (mean ± SD); 90.8 ± 3.4% (mean ± SD) with two columns.The purity of CD34 + cells varies due to the variation of human samples and experimental set-up. |
Before separation | |
MACSprep™ Chimerism CD34 MicroBead Kit, humanFigure 2Isolation of CD34 + cells from a whole blood sample from a healthy donor using the MACSprep™ Chimerism CD34 MicroBead Kit, two LS Columns, a MACSmix™ Tube Rotator and a MidiMACS™ Separator. Cells were fluorescently stained with CD34-FITC and CD45-VioBlue ® and analyzed by flow cytometry using the MACSQuant ® Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. The purity of CD34 + cells after separation is defined by percentage of CD34 + cells (viable CD45d im/+CD34 + cells) amongst leukocytes (viable CD45 dim/+ cells). The CD34 + cells content of the isolated fraction with one column is typically around 69.1 ± 5.8% (mean ± SD); 90.8 ± 3.4% (mean ± SD) with two columns.The purity of CD34 + cells varies due to the variation of human samples and experimental set-up. | |
After separation with one column | After separation with two columns |
MACSprep™ Chimerism CD34 MicroBead Kit, humanFigure 2Isolation of CD34 + cells from a whole blood sample from a healthy donor using the MACSprep™ Chimerism CD34 MicroBead Kit, two LS Columns, a MACSmix™ Tube Rotator and a MidiMACS™ Separator. Cells were fluorescently stained with CD34-FITC and CD45-VioBlue ® and analyzed by flow cytometry using the MACSQuant ® Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. The purity of CD34 + cells after separation is defined by percentage of CD34 + cells (viable CD45d im/+CD34 + cells) amongst leukocytes (viable CD45 dim/+ cells). The CD34 + cells content of the isolated fraction with one column is typically around 69.1 ± 5.8% (mean ± SD); 90.8 ± 3.4% (mean ± SD) with two columns.The purity of CD34 + cells varies due to the variation of human samples and experimental set-up. | MACSprep™ Chimerism CD34 MicroBead Kit, humanFigure 2Isolation of CD34 + cells from a whole blood sample from a healthy donor using the MACSprep™ Chimerism CD34 MicroBead Kit, two LS Columns, a MACSmix™ Tube Rotator and a MidiMACS™ Separator. Cells were fluorescently stained with CD34-FITC and CD45-VioBlue ® and analyzed by flow cytometry using the MACSQuant ® Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. The purity of CD34 + cells after separation is defined by percentage of CD34 + cells (viable CD45d im/+CD34 + cells) amongst leukocytes (viable CD45 dim/+ cells). The CD34 + cells content of the isolated fraction with one column is typically around 69.1 ± 5.8% (mean ± SD); 90.8 ± 3.4% (mean ± SD) with two columns.The purity of CD34 + cells varies due to the variation of human samples and experimental set-up. |
Isolation of CD34 + cells from a whole blood sample from a healthy donor using the MACSprep™ Chimerism CD34 MicroBead Kit, two LS Columns, a MACSmix™ Tube Rotator and a MidiMACS™ Separator. Cells were fluorescently stained with CD34-FITC and CD45-VioBlue ® and analyzed by flow cytometry using the MACSQuant ® Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. The purity of CD34 + cells after separation is defined by percentage of CD34 + cells (viable CD45d im/+CD34 + cells) amongst leukocytes (viable CD45 dim/+ cells). The CD34 + cells content of the isolated fraction with one column is typically around 69.1 ± 5.8% (mean ± SD); 90.8 ± 3.4% (mean ± SD) with two columns.The purity of CD34 + cells varies due to the variation of human samples and experimental set-up. |
Before separation | |
MACSprep™ Chimerism CD34 MicroBead Kit, humanFigure 2Isolation of CD34 + cells from a whole blood sample from a healthy donor using the MACSprep™ Chimerism CD34 MicroBead Kit, two LS Columns, a MACSmix™ Tube Rotator and a MidiMACS™ Separator. Cells were fluorescently stained with CD34-FITC and CD45-VioBlue ® and analyzed by flow cytometry using the MACSQuant ® Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. The purity of CD34 + cells after separation is defined by percentage of CD34 + cells (viable CD45d im/+CD34 + cells) amongst leukocytes (viable CD45 dim/+ cells). The CD34 + cells content of the isolated fraction with one column is typically around 69.1 ± 5.8% (mean ± SD); 90.8 ± 3.4% (mean ± SD) with two columns.The purity of CD34 + cells varies due to the variation of human samples and experimental set-up. | |
After separation with one column | After separation with two columns |
MACSprep™ Chimerism CD34 MicroBead Kit, humanFigure 2Isolation of CD34 + cells from a whole blood sample from a healthy donor using the MACSprep™ Chimerism CD34 MicroBead Kit, two LS Columns, a MACSmix™ Tube Rotator and a MidiMACS™ Separator. Cells were fluorescently stained with CD34-FITC and CD45-VioBlue ® and analyzed by flow cytometry using the MACSQuant ® Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. The purity of CD34 + cells after separation is defined by percentage of CD34 + cells (viable CD45d im/+CD34 + cells) amongst leukocytes (viable CD45 dim/+ cells). The CD34 + cells content of the isolated fraction with one column is typically around 69.1 ± 5.8% (mean ± SD); 90.8 ± 3.4% (mean ± SD) with two columns.The purity of CD34 + cells varies due to the variation of human samples and experimental set-up. | MACSprep™ Chimerism CD34 MicroBead Kit, humanFigure 2Isolation of CD34 + cells from a whole blood sample from a healthy donor using the MACSprep™ Chimerism CD34 MicroBead Kit, two LS Columns, a MACSmix™ Tube Rotator and a MidiMACS™ Separator. Cells were fluorescently stained with CD34-FITC and CD45-VioBlue ® and analyzed by flow cytometry using the MACSQuant ® Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. The purity of CD34 + cells after separation is defined by percentage of CD34 + cells (viable CD45d im/+CD34 + cells) amongst leukocytes (viable CD45 dim/+ cells). The CD34 + cells content of the isolated fraction with one column is typically around 69.1 ± 5.8% (mean ± SD); 90.8 ± 3.4% (mean ± SD) with two columns.The purity of CD34 + cells varies due to the variation of human samples and experimental set-up. |
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