The MACS
®
LENTIcheck Kit, human is a qPCR-based assay developed for the rapid assessment of replication competent lentivirus (RCL) in cell-based products transduced with lentiviral vectors provided by Lentigen and Miltenyi Biotec. The MACS LENTIcheck kit detects the lentiviral envelope gene sequences (vesicular stomatitis virus G glycoprotein [VSVg]). VSVg is thought to be the most suitable target for detecting RCL as incorporation of the envelope gene sequence is required to generate a replication competent virus. The absence of the VSV-G gene in ex vivo transduced cells according to the PCR assay can be used for lot release testing of cell-based products, in particular, when time

Specifications for
MACS
®
LENTIcheck Kit
, human

Overview

The MACS
®
LENTIcheck Kit, human is a qPCR-based assay developed for the rapid assessment of replication competent lentivirus (RCL) in cell-based products transduced with lentiviral vectors provided by Lentigen and Miltenyi Biotec. The MACS LENTIcheck kit detects the lentiviral envelope gene sequences (vesicular stomatitis virus G glycoprotein [VSVg]). VSVg is thought to be the most suitable target for detecting RCL as incorporation of the envelope gene sequence is required to generate a replication competent virus. The absence of the VSV-G gene in ex vivo transduced cells according to the PCR assay can be used for lot release testing of cell-based products, in particular, when time constrains are present.
Important note:
The primers included in the MACS LENTIcheck Kit, human are designed to amplify an amplicon of the lentiviral envelope gene sequence (VSVg). These primers are only compatible with the lentiviral vectors provided by Lentigen and Miltenyi Biotec.

Seems like you are coming from USA!
Do you want to visit our website in your country?