Benefits of light sheet microscopy

  • Fast and gentle 3D imaging
  • High frame rates
  • Minimum photodamage and fluorophore bleaching

High-speed imaging of fragile life specimens and large cleared tissue

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Samples are excited from the side by a focused light sheet while the fluorescence light is detected by a sCMOS camera perpendicular to the illumination plane. 3D image stacks are generated by moving the sample through the light sheet.

High frame rates

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Illuminating only the focal plane of the detection objective lens allows 3D microscopy utilizing a camera-based wide-field microscope with high frame rates.

Minimum photodamage and fluorophore bleaching

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As only the actually observed section is illuminated, photodamage and fluorophore bleaching are kept at a minimum.

UltraMicroscope II light sheet microscope

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