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Benefits of light sheet microscopy
Fast and gentle 3D imaging
High frame rates
Minimum photodamage and fluorophore bleaching
High-speed imaging of fragile life specimens and large cleared tissue
Samples are excited from the side by a focused light sheet while the fluorescence light is detected by a sCMOS camera perpendicular to the illumination plane. 3D image stacks are generated by moving the sample through the light sheet.
High-speed imaging of fragile life specimens and large cleared tissue
Illuminating only the focal plane of the detection objective lens allows 3D microscopy utilizing a camera-based wide-field microscope with high frame rates.
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