MSC Enumeration Kit, human

MSC Enumeration Kit, human

The MSC Enumeration Kit was developed for the standardized identification and quantification of human MSCs from fresh bone marrow aspirate by flow cytometry.
The MSC Enumeration Kit includes:
  • cocktail of three fluorochrome-conjugated antibodies including MSCs characterization marker CD271 (LNGFR) and exclusion markers CD45 and CD235a
  • additional MSC marker MSCA-1 (W8B2), for the identification of MSCs with highly proliferative potential
  • cocktail of four fluorochrome-conjugated isotype control antibodies
  • cell viability marker 7-AAD
  • Red Blood Cell Lysis Solution to remove erythrocytes
  • FcR Blocking Reagent to prevent unspecific binding

Background information

The success of bone marrow (BM)-derived mesenchymal stem cell (MSC) experiments depends on the quality of bone marrow aspirate (BMA), as well as the number of MSCs in the BMA sample. Commonly, MSC quantification is performed by CFU-F (colony-forming unit fibroblast) assay, which are time consuming and highly variable.
Based on the finding that the number of CFU-F colonies generated after 14 days of BM MNC (bone marrow mononuclear cell) culture is linearly related to the number of CD271
bright
cells in BMA
1
, we’ve developed a reliable procedure for absolute quantification of MSCs in BMA.
Product options: 1

Product information

Size
Order no.
Price

MSC Enumeration Kit, human

  • For research use only
for 25
tests
130-106-646
EUR 566,00 

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