CD4+ RTE Enumeration Kit, human

CD4
+
RTE Enumeration Kit
, human

The CD4
+
Recent Thymic Emigrant Enumeration Kit is specifically designed for the analysis of CD4
+
CD31
+
CD62L
+
CD45RO
T cells (recent thymic emigrants, RTEs), enabling an easy and reliable approach to the quantification of human thymic naive T cells.
The kit contains all necessary reagents for the identification and enumeration of CD4
+
RTEs by flow cytometry.

Background information

RTEs are a subpopulation of naive T cells characterized by their content of T cell receptor excision circles (TRECs) — stable DNA episomes formed during T cell receptor rearrangement. TRECs are not replicated during mitosis and dilute out with each cellular division. The determination of a cell’s TREC content has been used for the quantitation of thymic T cell production. The output of RTEs from the thymus and the homeostatic proliferation of T cells comprise the total naive T cell pool.
CD4
+
RTEs play a critical role in immunoreconstitution, for example after bone marrow transplantation or chemotherapy where the repopulation of the T cell repertoire is essential. The number of CD4
+
RTEs is thought to be an indicator for the recovery of the immune system and provides information on thymic function.
The expression of CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) on CD4
+
RTEs enables enumeration of CD4
+
RTEs by flow cytometry, providing an alternative to the time-consuming PCR-based characterization of CD4
+
RTE TREC content. The identification of CD4
+
RTEs is possible through analysis for their expression of CD31, CD45RA, and CD62L, and a lack of CD45RO expression. CD4
+
T cells lacking CD31 expression harbor little or no TRECs, suggesting that they already proliferated in the periphery.
1,2,3
Product options: 2

Product information

Size
Order no.
Price

CD4
+
RTE Enumeration Kit
, human

  • For research use only
for 50
tests
(1)
130-092-055
EUR 974,00 

CD4
+
RTE Enumeration Kit
, human

  • For research use only
for 2×50
tests
(1)
130-092-056
EUR 1.357,00 

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