CD154/IL-4/CD4 Detection Kit, human

CD154/IL-4/CD4 Detection Kit, human

The kit is suitable for flow cytometric identification and enumeration of IL-4-producing CD154
+
CD4
+
T cells upon
in vitro
stimulation. Other applications include identification and enumeration of IL-4-producing, antigen-specific CD154
+
CD4
+
T cells upon restimulation or monitoring of specificity of antigen-specific T cell lines.

Background information

The CD154/IL-4/CD4 Detection Kit is designed for the intracellular detection of IL-4 production in activated CD4
+
T cells. It contains an antibody recognizing CD154 – a specific marker for T cell activation – and all reagents necessary for the immunofluorescent detection of IL-4-producing activated CD4
+
T cells from whole blood or PBMCs.
By combining the detection of cytokine production with staining of CD154 and CD4, the sensitive and specific detection of activated antigen-specific CD4
+
T cells is easily possible.
IL-4 is predominantly secreted by activated CD4
+
and CD4
+
effector and memory T cells, basophils, and mast cells. IL-4 especially induces and supports humoral responses, e.g., by its effects on the activation, proliferation, and antibody production of B cells.
Product options: 1

Product information

Size
Order no.
Price

CD154/IL-4/CD4 Detection Kit, human

  • For research use only
for 100
tests
(1)
130-092-817
EUR 1.122,00