MACSQuant®
Analyzer 16
Flow Cytometer

Colorful new possibilities in automated flow cytometry

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Benefits

Full automation

Load your samples, import your experiment settings, and leave the staining, data acquisition and analysis to the MACSQuant Analyzer 16

Expanded fluorescent channels

Amplify your data with a selection from 14 fluorescence channels and an average of 40% less sample volume required.

Flexible assay setup

Easily and immediately switch between tubes and plates at the click of a button.

Multi-instrument alignment

Harmonize data with collaborating labs to ensure reproducibility.

Features & specifications

MACSQuant Device Front

Large monitor:

15.6" monitor for simple touchscreen operation

Robotic needle arm

At the heart of sample mixing, processing and autolabeling

Integrated MACS® Magnetic Cell Enrichmentt:

Focus on your cells of interest and fortify your data

Universal Reagent Rack:

Flexible autolabeling options from glass or plastic vials

     

Large monitor:

15.6" monitor for simple touchscreen operation

Robotic needle arm

At the heart of sample mixing, processing and autolabeling

Integrated MACS® Magnetic Cell Enrichment:

Focus on your cells of interest and fortify your data

Universal Reagent Rack:

Flexible autolabeling options from glass or plastic vials

MiniSampler

MACS® MiniSampler Plus:

Seamless sampling from single tubes, multiple tubes or 96-well plate

MACSQuant Device Front
Optics
Laser excitationSpatially separated:
405 nm, 65 mW diode
488 nm, 50 mW DPSS
(diode pumped solid state)
640 nm, 72 mW diode
Emission detectorsFSC: 488/10 nm

SSC: 405/10 nm

B1: 525/50 nm
B2: 579/34 nm
B3: 615/20 nm
B4: 667/30 nm
B5: 725/40 nm
B6: 785/62 nm
V1: 450/50 nm
V2: 525/50 nm
V3: 579/34 nm
V4: 615/20 nm
V5: 667/30 nm
R1: 667/30 nm
R2: 725/40 nm
R3: 785/62 nm

Fluorescence sensitivity (MESF)FITC < 110
PE < 75
APC < 100
Fluorescence precision (CV)< 5% CV with alignment verification particles
Scatter resolutionScatter performance is optimized for resolving human peripheral blood lymphocytes,
monocytes, and granulocytes
Flow cell dimensions200×250 μm
Fluorescence detectorsOptimized with spectrally matched PMTs for all channels 
Optical alignmentFixed tree-like configuration, no user adjustments needed
Laser spot size15×45 μm
Fluidics
Minimal measurement volume1 μL (25 μL for full spectrum)
Dead volume10 μL
Sample flow rate25–100 μL/minute plus automated flow rate to maintain 500, 1,000, or 2,000 events/second
Measurement speed1,425 minutes per 96-well plate (5 μL measurement volume; screen mode)
Sample uptakeVia robotic needle arm
Maximal event rate15,000 events/second
System maintenanceAutomated startup, PMT calibration, cleaning cycles, and shutdown
Sample mixingAspiration
Performance
Absolute counts performance
(reproducibility)1,2
CV < 7% 
Sample carryover1,30.01% (extended washing)
Sample tube/plates allowed96-well plate (U, V, flat, deep well) FACS tubes (5 mL) Eppendorf tubes
MACS® Cell Enrichment UnitFor pre-analysis enrichment of rare cells
1 Referred value indicates the average of multiple experiments and can differ for individual sample values.
2 For counting performance, full 96-well plates were loaded with 200 μL/well of peripheral blood mononuclear cell (PBMC) suspension at a nominal concentration
of 5000 cells/μL. The uptake volume was set to 50 μL at medium flowrate.
3 For carry-over, full 96-well plates were loaded with 200 μL/well of PBMC suspension at a nominal concentration of 5000 cells/μL in every other well (“SRC-wells”).
Alternating wells are loaded with an equal volume of MACSQuant® Running Buffer (“CO-wells”). The uptake volume was set to 50 μL at medium flowrate.
The carry-over is defined by sum(CO-singlet count)/sum(SRC-singlet count) ×100%.
4 The measurement speed is determined by measuring the time between the first movement of the robotic arm into the first measured well and the first
movement out of the last well. The measurements itself were carried out at the highest possible flow rate in fast mode measuring 5 μL per well.

Download the brochure for more information

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Applications

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Webinar

Learn about multicolor flow cytometry on the MACSQuant Analyzer 16

Software

Harmonizing the world is just an instrument setting away with MACSQuantify™ Software

  • Easy assay transfer from one instrument to another for comparable, standardized results and multi-instrument alignment during inter-lab collaborations
  • Express modes with predefined experiment settings as well as acquisition and analysis templates 
  • Automated fluorescence compensation
  • Compliance with 21 CFR Part 11 for data submission to regulatory agencies
  • Audit trail, analysis reports with e-singature, and user management system according to 21 CFR Part 11
See all our software solutions

Support

Support at your fingertips

  • MACSQuant Live Support for real-time assistance from our experts
  • Technical and field application support for assay design, custom automation, and express mode development
  • Instrument training at regional MACS Academy Miltenyi Biotec facilities
  • Comprehensive service options provided by our globally distributed field service teams

MACSQuant®
Analyzer 16
Flow Cytometer

Colorful new possibilities in automated flow cytometry. Order no. 130-109-803. For research use only.

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Miltenyi Biotec B.V.
Schipholweg 68 H
2316 XE Leiden
The Netherlands

Phone: 0800 94016
Fax: 0800 99626
E-Mail: macs@miltenyibiotec.nl
Web: www.miltenyibiotec.com

Technical support - Clinical products

Phone:
Fax:
E-Mail: macs@miltenyibiotec.nl