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Technical support - Clinical products
|Laser excitation||Spatially separated:|
405 nm, 65 mW diode
488 nm, 50 mW DPSS
(diode pumped solid state)
640 nm, 72 mW diode
|Emission detectors||FSC: 488/10 nm|
SSC: 405/10 nm
B1: 525/50 nm
|Fluorescence sensitivity (MESF)||FITC < 110|
PE < 75
APC < 100
|Fluorescence precision (CV)||< 5% CV with alignment verification particles|
|Scatter resolution||Scatter performance is optimized for resolving human peripheral blood lymphocytes,|
monocytes, and granulocytes
|Flow cell dimensions||200×250 μm|
|Fluorescence detectors||Optimized with spectrally matched PMTs for all channels|
|Optical alignment||Fixed tree-like configuration, no user adjustments needed|
|Laser spot size||15×45 μm|
|Minimal measurement volume||1 μL (25 μL for full spectrum)|
|Dead volume||10 μL|
|Sample flow rate||25–100 μL/minute plus automated flow rate to maintain 500, 1,000, or 2,000 events/second|
|Measurement speed1,4||25 minutes per 96-well plate (5 μL measurement volume; screen mode)|
|Sample uptake||Via robotic needle arm|
|Maximal event rate||15,000 events/second|
|System maintenance||Automated startup, PMT calibration, cleaning cycles, and shutdown|
|Absolute counts performance|
|CV < 7%|
|Sample carryover1,3||0.01% (extended washing)|
|Sample tube/plates allowed||96-well plate (U, V, flat, deep well) FACS tubes (5 mL) Eppendorf tubes|
|MACS® Cell Enrichment Unit||For pre-analysis enrichment of rare cells|
|1 Referred value indicates the average of multiple experiments and can differ for individual sample values.|
2 For counting performance, full 96-well plates were loaded with 200 μL/well of peripheral blood mononuclear cell (PBMC) suspension at a nominal concentration
of 5000 cells/μL. The uptake volume was set to 50 μL at medium flowrate.
3 For carry-over, full 96-well plates were loaded with 200 μL/well of PBMC suspension at a nominal concentration of 5000 cells/μL in every other well (“SRC-wells”).
Alternating wells are loaded with an equal volume of MACSQuant® Running Buffer (“CO-wells”). The uptake volume was set to 50 μL at medium flowrate.
The carry-over is defined by sum(CO-singlet count)/sum(SRC-singlet count) ×100%.
4 The measurement speed is determined by measuring the time between the first movement of the robotic arm into the first measured well and the first
movement out of the last well. The measurements itself were carried out at the highest possible flow rate in fast mode measuring 5 μL per well.